Results 141 to 150 of about 3,773 (182)

Polynucleotide ligase from rat liver after partial hepatectomy

Biochemical and Biophysical Research Communications, 1971
Abstract Polynucleotide ligase, which catalyzes the covalent joining of two segments of an interrupted strand in a DNA duplex, was extracted from rat liver. The activity is located in both nuclei and soluble fractions. Its optimal pH was 8.0. The enzyme requires ATP as a cofactor. Its activity was completely dependent on the presence of Mg 2+ .
K, Tsukada, M, Ichimura
openaire   +2 more sources

Purification and properties of a polynucleotide ligase from calf thymus glands

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
Abstract Polynucleotide ligase has been purified over 390-fold from extracts of calf thymus glands. A solid-state model of DNA containing single-strand breaks, namely, the poly(dA) · [poly([ 3 H]dT)-poly(dT)]-cellulose, was used as substrate for the enzymatic assays.
BERTAZZONI, Umberto, M. Mathelet, F. Campagnari   +2 more
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Changes in polynucleotide ligase during rat liver regeneration

Biochemical and Biophysical Research Communications, 1974
Abstract The specific activity of polynucleotide ligase in rat liver seems to begin to rise at 16 hours after partial hepatectomy (removal of 70% of the liver). The increases reach their maxima about 24 hours after operation, rising to at least 4 to 5 fold normal levels.
openaire   +2 more sources

Polynucleotide ligase in mouse cells infected by polyoma virus

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
Abstract 1. The properties of polynucleotide ligase from mouse cells infected with polyoma virus, and from uninfected mouse cells, were studied in order to investigate the possible involvement of ligase in polyoma DNA replication. 2. Two procedures for the assay of polynucleotide ligase were used to detect ligase in extracts of mouse embryo cells ...
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On the fidelity of phage T4-induced polynucleotide ligase in the joining of chemically synthesized deoxyribooligonucleotides

Biochemical and Biophysical Research Communications, 1970
Abstract The fidelity of phage T 4 -induced polynucleotide ligase has been studied by using chemically synthesized deoxyribooligonucleotide p 3 2 T 1 2 and p 3 2 T 1 1 C. In the presence of polydeoxyadenylate,each substrate is joined by ligase to form longer chain deoxyribopolynucleotides.
Tsiapalis, C., Narang, S.
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T₄ polynucleotide ligase catalyzed joining on triple-stranded nucleic acids

Biochemistry, 1978
dT1O will form triple-stranded complexes with dAn and these complexes can serve as substrate for T4 polynucleotide ligase (EC 6.5.1.1). The rate of phosphodiester formation was found to be approximately the same as for the double-stranded complex and, furthermore, the rate appears to be similar on the two strands in the complex.
A J, Raae, K, Kleppe
openaire   +2 more sources

Role of polynucleotide ligase in T4 DNA replication

Journal of Molecular Biology, 1968
J, Newman, P, Hanawalt
openaire   +2 more sources

Polynucleotide Ligase from Cultured Plant Cells

The Journal of Biochemistry, 1971
K, Tsukada, A, Nishi
openaire   +2 more sources

Repair of single strand breaks in transforming DNA by polynucleotide ligase

Biochemical and Biophysical Research Communications, 1968
J, Takagi, T, Ando, Y, Ikeda
openaire   +2 more sources

E3 ubiquitin ligases: styles, structures and functions

Molecular Biomedicine, 2021
Yang Wang
exaly