Results 161 to 170 of about 3,904 (190)
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Induction of polynucleotide ligase in human lymphocytes stimulated by phytohemoagglutinin
Biochemical and Biophysical Research Communications, 1972Abstract The treatment of human lymphocytes with phytohemoagglutinin causes the appearance of the activity of polynucleotide ligase, rising at least 50 fold from levels below the background. This increase takes place in the fourth or fifth day after treatment, and is delayed by one day approximately with respect to the rise of DNA synthesis rate; the
A M, Pedrini +4 more
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The activity of mammalian polynucleotide ligase on X-irradiated DNAs
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978Selected samples of heterogeneous DNA from calf thymus with similar number-average molecular weight, Mn, and a low incidence of single-strand breaks were exposed in aqueous solutions to a mild X-ray dose of 1500 rads. The irradiation produced on the average about 0.2 bihelical and 2.2 monohelical scissions per DNA molecule of 1708 000 Mn.
M, Mathelet +3 more
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Role of polynucleotide ligase and DNA polymerase in the repair of DNA damage
Journal of Molecular Biology, 1969 K. Ebisuzaki
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Purification and properties of a polynucleotide ligase from calf thymus glands
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract Polynucleotide ligase has been purified over 390-fold from extracts of calf thymus glands. A solid-state model of DNA containing single-strand breaks, namely, the poly(dA) · [poly([ 3 H]dT)-poly(dT)]-cellulose, was used as substrate for the enzymatic assays.
BERTAZZONI, Umberto +2 more
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The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases
Current Opinion in Structural Biology, 2004ATP- and NAD(+)-dependent DNA ligases, ATP-dependent RNA ligases and GTP-dependent mRNA capping enzymes comprise a superfamily of proteins that catalyze nucleotidyl transfer to polynucleotide 5' ends via covalent enzyme-(lysyl-N)-NMP intermediates. The superfamily is defined by five peptide motifs that line the nucleotide-binding pocket and contribute ...
Stewart, Shuman, Christopher D, Lima
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T4 polynucleotide ligase catalyzed joining on triple-stranded nucleic acids
Biochemistry, 1978dT1O will form triple-stranded complexes with dAn and these complexes can serve as substrate for T4 polynucleotide ligase (EC 6.5.1.1). The rate of phosphodiester formation was found to be approximately the same as for the double-stranded complex and, furthermore, the rate appears to be similar on the two strands in the complex.
A J, Raae, K, Kleppe
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Chemico-Biological Interactions, 1981
Conformationally distinct chromatin populations were utilized as substrates to quantitate the relative amount of and accessibility of internal 5'-phosphomonoester breaks in DNA-chromatin. In these studies, a constant amount of chromatin as well as deproteinized DNA derived from the respective chromatin sample was titrated with increasing quantities of ...
R A, Montagna +3 more
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Conformationally distinct chromatin populations were utilized as substrates to quantitate the relative amount of and accessibility of internal 5'-phosphomonoester breaks in DNA-chromatin. In these studies, a constant amount of chromatin as well as deproteinized DNA derived from the respective chromatin sample was titrated with increasing quantities of ...
R A, Montagna +3 more
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Polynucleotide Ligase from Cultured Plant Cells
The Journal of Biochemistry, 1971K, Tsukada, A, Nishi
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Role of polynucleotide ligase in T4 DNA replication
Journal of Molecular Biology, 1968J, Newman, P, Hanawalt
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Alkaline phosphatase, T4 polynucleotide kinase, T4 DNA ligase
2007Alkalna fosfataza katalizira reakciju uklanjanja fosfatne skupine. DNA i RNA molekule na 5’ kraju posjeduju fosfatnu skupinu. T4 polinukleotid kinaza katalizira prijenos γ -fosfata s ATP-a na 5' OH skupinu jednolančane ili dvolančane DNA ili RNA i nukleotid 3' monofosfata. T4 DNA ligaza je enzim koji se koristi za spajanje (ligaciju) odsječaka DNA.
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