Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources
Distinct co-evolution patterns of genes associated to DNA polymerase III DnaE and PolC
BMC Genomics, 2012 Background Bacterial genomes displaying a strong bias between the leading and the lagging strand of DNA replication encode two DNA polymerases III, DnaE and PolC, rather than a single one.
Engelen Stefan +3 more
doaj +1 more source
Proteomic alterations of Escherichia coli by paraquat [PDF]
, 2010 Paraquat (PQ; a widely used herbicide) exerts its harmful effect to human, mammals and microorganisms upon intracellular conversion to superoxide radical.
Isarankura-Na-Ayudhya, Chartchalerm +7 more
core
miRNAs as Influencers of Cell-Cell Communication in Tumor Microenvironment [PDF]
, 2020 microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level, inducing the degradation of the target mRNA or translational repression.
Conti, Ilaria +6 more
core +1 more source
Antibiotic influx and efflux in Pseudomonas aeruginosa: Regulation and therapeutic implications
Microbial Biotechnology, Volume 17, Issue 5, May 2024.This work comprehensively summarized the regulatory networks of the major porin proteins and efflux pumps that play critical roles in antibiotic influx and efflux in Pseudomonas aeruginosa. It also discussed promising therapeutic approaches to combat multidrug‐resistant P.
Weiyan Wu, Jiahui Huang, Zeling Xu
wiley +1 more source
The biological essence of synthetic lethality: Bringing new opportunities for cancer therapy
MedComm – Oncology, Volume 3, Issue 1, March 2024.Synthetic lethality exploits vulnerabilities in cancer cells, targeting aberrant pathways resulting from genetic mutations or pharmacological interventions. This strategy extends beyond the tumor cells to include the tumor microenvironment, such as stromal cells and signaling molecules, to enhance treatment responses and eradicate cancer.
Meiyi Ge +4 more
wiley +1 more source
Kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase
Journal of Biological Chemistry, 1975 The kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of Micrococcus luteus has been investigated. Double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either ...
J Y, Chou, M F, Singer, P, McPhie
openaire +2 more sources
The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8 [PDF]
, 2014 BACKGROUND: RNA metabolism, including RNA synthesis and RNA degradation, is one of the most conserved biological systems and has been intensively studied; however, the degradation network of ribonucleases (RNases) and RNA substrates is not fully ...
Akeo Shinkai +7 more
core +1 more source
Protein Science, Volume 33, Issue 2, February 2024.Abstract Escherichia coli is one of the most widely utilized hosts for production of recombinant membrane proteins (MPs). Bacterial MP production, however, is usually accompanied by severe toxicity and low‐level volumetric accumulation. In previous work, we had discovered that co‐expression of RraA, an inhibitor of the RNA‐degrading activity of RNase E,
Eleni Vasilopoulou +2 more
wiley +1 more source
PNPase knockout results in mtDNA loss and an altered metabolic gene expression program.
PLoS ONE, 2018 Polynucleotide phosphorylase (PNPase) is an essential mitochondria-localized exoribonuclease implicated in multiple biological processes and human disorders.
Eriko Shimada +11 more
doaj +1 more source