Results 211 to 220 of about 225,549 (221)

A New Member of Family 11 Polysaccharide Lyase, Rhamnogalacturonan Lyase (CtRGLf) from Clostridium thermocellum

Molecular Biotechnology, 2016
A thermostable, alkaline rhamnogalacturonan lyase (RG lyase) CtRGLf, of family 11 polysaccharide lyase from Clostridium thermocellum was cloned, expressed, purified and biochemically characterised. Both, the full-length CtRGLf (80 kDa) protein and its truncated derivative CtRGL (63.9 kDa) were expressed as soluble proteins and displayed maximum ...
Arun, Dhillon, Vania O, Fernandes, Fernando M V, Dias, José A M, Prates, Luis M A, Ferreira, Carlos M G A, Fontes, M S J, Centeno, Arun, Goyal   +7 more
openaire   +2 more sources

The Swiss Army Knife of Alginate Metabolism: Mechanistic Analysis of a Mixed-Function Polysaccharide Lyase/Epimerase of the Human Gut Microbiota.

Journal of the American Chemical Society
The alginate-degrading enzyme BoPL38 of the human gut bacterium Bacteroides ovatus CP926 degrades all three polysaccharide structures found in alginate, a major constituent of brown macroalgae, making it a valuable tool for the selective production of ...
T. Tandrup, José Pablo Rivas-Fernández, Mikkel Madsen, Mette E. Rønne, Agnes B Petersen, L. Klau, A. Tøndervik, C. Wilkens, F. Aachmann, Carme Rovira, Birte Svensson   +10 more
semanticscholar   +1 more source

Ulvan polysaccharide-degrading enzymes: An updated and comprehensive review of sources category, property, structure, and applications of ulvan lyases

Algal Research, 2021
Tiancheng Tang, Shengsheng Cao, Benwei Zhu, Qian Li   +3 more
semanticscholar   +1 more source

An Ancestral Member of the Polysaccharide Lyase Family 2 Displays Endolytic Activity and Magnesium Dependence

Applied Biochemistry and Biotechnology, 2013
Polysaccharide lyases (PLs) are enzymes that cleave glycosidic linkages in hexuronate polysaccharides, such as homogalacturonan (HG), using a β-elimination mechanism. Traditionally, PL activities on HG have been associated with catalytic calcium cofactors, unusually high pH optima, and arginine Brønstead bases.
D Wade, Abbott, Dallas, Thomas, Benjamin, Pluvinage, Alisdair B, Boraston   +3 more
openaire   +2 more sources

On the catalytic mechanism of polysaccharide lyases: evidence of His and Tyr involvement in heparin lysis by heparinase I and the role of Ca2+.

Molecular Biosystems, 2014

semanticscholar   +1 more source

Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases.

Archives of Biochemistry and Biophysics, 1999
W. Hashimoto, E. Kobayashi, H. Nankai, N. Sato, T. Miya, S. Kawai, K. Murata   +6 more
semanticscholar   +1 more source

The synthesis of a novel thio-linked disaccharide of chondroitin as a potential inhibitor of polysaccharide lyases.

Carbohydrate Research, 2004
C. Rye, S. Withers
semanticscholar   +1 more source

A marine bacterial enzymatic cascade degrades the algal polysaccharide ulvan

Nature Chemical Biology, 2019
L. Reisky, Aurélie Préchoux, M. Zühlke, Marcus Bäumgen, C. Robb, N. Gerlach, T. Roret, Christian Stanetty, R. Larocque, G. Michel, Tao Song, Stephanie Markert, F. Unfried, M. Mihovilovic, Anke Trautwein-Schult, D. Becher, T. Schweder, U. Bornscheuer, J. Hehemann   +18 more
semanticscholar   +1 more source

Comparative molecular dynamics simulations provided insights into the mechanisms of cold-adaption of alginate lyases from the PL7 family

Extremophiles
Fei Bian, Xiao-Yue Liang, Meng Wang, Zhong-Zhi Sun, Bin-Bin Xie   +4 more
semanticscholar   +1 more source

Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity

Marine Biotechnology
Xue Li, Miao Yang, Kun-lian Mo, Yong-hua Hu, Han-jie Gu, Dong-mei Sun, S. Bao, Hui-qin Huang   +7 more
semanticscholar   +1 more source