Results 51 to 60 of about 102,030,339 (160)

Droplet Digital CRISPR for Nucleic Acid Detection

open access: yesAdvanced Science, Volume 13, Issue 14, 9 March 2026.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang   +4 more
wiley   +1 more source

First Molecular Detection of Torque Teno Canis Virus in Apparently Healthy Dogs in Southern Italy

open access: yesVeterinary Medicine and Science, Volume 12, Issue 1, January 2026.
•Torque teno canis virus has been detected in dogs from southern Italy. •Detection of TTCaV DNA was more frequent in dogs with an altered faecal score, mixed‐breed and belonging to kennels. ABSTRACT Background Torque teno viruses are viruses with unclear pathogenic potential, as conflicting evidence has classified this family of viruses as harmless and
Gianmarco Ferrara   +3 more
wiley   +1 more source

Field Evaluation of a Novel Combined Vaccine Against Porcine Circovirus Types 2a/d, Mycoplasma hyopneumoniae and Mycoplasma hyorhinis With an Emphasis on Growth Performance

open access: yesVeterinary Medicine and Science, Volume 12, Issue 1, January 2026.
A field trial on three commercial farms evaluated a combined vaccine targeting PCV2a/d, Mycoplasma hyopneumoniae, and Mycoplasma hyorhinis. Vaccinated pigs showed improved average daily weight gain, stronger immune responses against these pathogens, and significantly reduced viral and mycoplasmal loads and lesion severity, supporting its efficacy under
Jeongmin Suh   +7 more
wiley   +1 more source

Development of a novel PCV2 and PCV3 vaccine using virus-like vesicles incorporating Venezuelan equine encephalomyelitis virus-containing vesicular stomatitis virus glycoprotein

open access: yesFrontiers in Veterinary Science
Porcine circovirus disease (PCV) causes substantial economic losses in the pig industry, primarily from porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3). Novel vaccines are necessary to prevent and control PCV infections.
Ying Wang   +8 more
doaj   +1 more source

Exploratory field study on the effects of porcine circovirus 2 (PCV-2) sow vaccination at different physiological stages mimicking blanket vaccination

open access: yesPorcine Health Management, 2021
Background The objective of the present study was to explore the benefits of Porcine circovirus 2 (PCV-2) blanket vaccination in a sow herd on productive parameters, PCV-2 infection and immune status in sows and their progeny.
Patricia Pleguezuelos   +9 more
doaj   +1 more source

Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production

open access: yesPathogens, 2022
Vertical transmission is a consistently discussed pathway of porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3) transmission in pigs.
Matthias Eddicks   +10 more
doaj   +1 more source

A Rapid p72‐Targeting Colloidal Gold Immunochromatographic Strip for African Swine Fever Virus Detection

open access: yesTransboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
African swine fever (ASF) is a lethal, hemorrhagic, and highly contagious infectious disease caused by the African swine fever virus (ASFV). At present, ASFV has evolved into multiple variant strains with different levels of virulence in China, complicating the epidemiological landscape.
Wan Wang   +14 more
wiley   +1 more source

Development and Application of a Rep‐Targeted SYBR Green I qPCR Assay for Porcine Circovirus 4: Etiological Investigation in China (2022–2025) and Molecular Evolutionary Analysis

open access: yesTransboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
Porcine circovirus (PCV) type 4 (PCV4) is an emerging Circovirus whose epidemiological and evolutionary dynamics remain incompletely characterized. We developed a replication‐associated protein (Rep)‐targeted SYBR Green I real‐time quantitative PCR assay that showed analytical specificity against tested common porcine pathogens and an estimated 95 ...
Yi Lu   +10 more
wiley   +1 more source

Establishment and Evaluation of a Multicolor Latex Microsphere‐Based Lateral Flow Immunoassay for the Simultaneous Detection of Antibodies Against African and Classical Swine Fever Viruses

open access: yesTransboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
African swine fever (ASF), a highly fatal disease often termed the “number one killer” of pigs, presents clinical symptoms indistinguishable from classical swine fever (CSF), such as fever, diarrhea, and vomiting, complicating on‐site differential diagnosis.
Jie Chen   +13 more
wiley   +1 more source

A RNase H2‐Linked TaqMan‐MGB Quantitative Real‐Time PCR Assay for Differential Detection of Mycoplasma hyopneumoniae Live‐Attenuated Vaccine Strains

open access: yesTransboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
Mycoplasma hyopneumoniae (M. hyopneumoniae), the etiological agent of porcine enzootic pneumonia, is one of the most prevalent and economically significant respiratory pathogens in the swine industry. Vaccination constitutes a primary strategy for controlling M. hyopneumoniae infections.
Kangyao Peng   +8 more
wiley   +1 more source

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