Porcine hemagglutinating encephalomyelitis virus (PHEV), which causes porcine encephalomyelitis and is widespread among swine worldwide. RNA interference (RNAi) pathways have emerged as important regulators of virus-host cell interactions. In this study, two siRNA expression plasmids (shN1 and shN2) were generated to target two different coding regions
Lan, Yungang +6 more
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2023 ACVIM Forum Research Abstract Program
Journal of Veterinary Internal Medicine, Volume 38, Issue 1, Page 530-729, January/February 2024.
wiley +1 more source
The PERK/PKR-eIF2α Pathway Negatively Regulates Porcine Hemagglutinating Encephalomyelitis Virus Replication by Attenuating Global Protein Translation and Facilitating Stress Granule Formation. [PDF]
Shi J +11 more
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Experimental infection on pigs with porcine hemagglutinating encephalomyelitis virus.
T, Hirahara +7 more
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Additional file 1: N2a cells infected with PHEV were treated with RES Virus inactivation assay. A Schematic diagrams illustrating the experimental design for time-of-addition experiments. B Expression of PHEV mRNA in N2a cells at 24 h as determined by qPCR assay. PHEV mRNA levels were quantified and normalized to GAPDH levels, respectively.
Liu, Yuzhu +5 more
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Additional file 2: N2a cells were pre-treated with 10 μM RES for 12 h at 37 °C with 5% CO2 before viral adsorption. Next, the culture medium containing the drug was removed then cells were inoculated with PHEV at 100 TCID50 (106.125) for 72 h at 37 °C with 5% CO2.
Liu, Yuzhu +5 more
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Pathogenicity of Porcine Hemagglutinating Encephalomyelitis Virus for Mouse and Guinea Pig.
Tadashi HIRAHARA +10 more
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Development of a triplex real-time RT-PCR assay for detection and differentiation of three US genotypes of porcine hemagglutinating encephalomyelitis virus. [PDF]
Wang L +7 more
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Porcine Hemagglutinating Encephalomyelitis Virus Activation of the Integrin α5β1-FAK-Cofilin Pathway Causes Cytoskeletal Rearrangement To Promote Its Invasion of N2a Cells. [PDF]
Lv X +11 more
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Serological Survey of Porcine Hemagglutinating Encephalomyelitis Virus Infection of Pigs in Japan
TADASHI HIRAHARA +8 more
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