Results 81 to 90 of about 4,410 (216)

Genomic characterization and high prevalence of bocaviruses in swine. [PDF]

PLoS ONE, 2011
Using random PCR amplification followed by plasmid subcloning and DNA sequencing, we detected bocavirus related sequences in 9 out of 17 porcine stool samples.
Tongling Shan, Daoliang Lan, Linlin Li, Chunmei Wang, Li Cui, Wen Zhang, Xiuguo Hua, Caixia Zhu, Wei Zhao, Eric Delwart   +9 more
doaj   +1 more source

Recent advances in the construction of humanized animal models and applications in translational medicine

Animal Models and Experimental Medicine, EarlyView.
Substantial advances have been achieved in the development of humanized mouse models, which have proven highly valuable in evaluating cancer immunotherapies and elucidating the mechanisms of infectious diseases. There is now a growing shift in research toward larger animal models—such as pigs—that offer greater physiological similarity to humans ...
Yanan Lyu, Yong‐Guang Yang, Zheng Hu
wiley   +1 more source

Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses

Viruses, 2018
In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues.
Vanessa M. Pfankuche, Kerstin Hahn, Rogier Bodewes, Florian Hansmann, André Habierski, Ann-Kathrin Haverkamp, Stephanie Pfaender, Stephanie Walter, Christine Baechlein, Alexander Postel, Eike Steinmann, Paul Becher, Albert Osterhaus, Wolfgang Baumgärtner, Christina Puff   +14 more
doaj   +1 more source

Establishment of Interspecies Somatic Cell Nuclear Transfer and Transgene‐Free Inducible Pluripotent Stem Cells for Versatile Conservation of the Germplasm Resource of Wild Boar

Animal Research and One Health, EarlyView.
Conserving genetic material and even increasing genetic diversity is critical. To conduct the conservation of wild boar germplasm resources, we have successfully obtained healthy cloned wild boars for the first time using interspecies somatic cell nuclear transfer and established transgene‐free iPSCs that can be used to conduct iterative rounds of gene
Chen Gao, Xinyi Zhou, Shigang Gu, Jianbo Li, Jing Wang, Yurong Zhang, Wenxuan Zhao, Xinhua Wei, Liting Lu, Quanmin Zhao, Yongye Huang, Dawei Yu   +11 more
wiley   +1 more source

Genetic analysis of porcine H3N2 viruses originating in southern China

Journal of General Virology, 1995
From immunological and phylogenetic analyses of H3 influenza viruses isolated from pigs and ducks in the People's Republic of China (China), Hong Kong, Taiwan and Japan, between 1968 and 1982, we arrived at the following conclusions. The H3 haemagglutinin and N2 neuraminidase genes from swine isolates can be segregated into four mammalian lineages ...
Nerome, K, Kanegae, Y, Shortridge, KF, Sugita, S, Ishida, M   +4 more
openaire   +3 more sources

The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding

Animal Research and One Health, EarlyView.
A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang, Weiran Wu, Qingqing Cai, Jiaxing Wang, Qi Gao, Muhammad Iftikhar, Yulin Bi, Hao Bai, Lihong Gu, Guobin Chang, Shihao Chen   +10 more
wiley   +1 more source

Sensitive detection systems for infectious agents in xenotransplantation*

Xenotransplantation, EarlyView., 2020
Abstract Xenotransplantation of pig cells, tissues, or organs may be associated with transmission of porcine microorganisms, first of all of viruses, to the transplant recipient, potentially inducing a disease (zoonosis). I would like to define detection systems as the complex of sample generation, sample preparation, sample origin, time of sampling ...
Joachim Denner
wiley   +1 more source

Virome of post-weaned diarrhoeic pigs and healthy cohorts in England

Virology Journal
Background Post-weaning diarrhoea (PWD) is a disease syndrome that negatively impacts pig health, welfare and productivity. PWD typically occurs within two weeks of weaning and coincides with significant physiological changes, including villus atrophy ...
Akbar Dastjerdi, Hannah Davies, Manal Abu Oun, Indre Navickaite, Siva Karuna, Mandy Nevel, Arianna Comin, Susanna Williamson   +7 more
doaj   +1 more source

Evolution of Porcine Virus Isolation: Guidelines for Practical Laboratory Application

Microorganisms
Cell cultures are an essential tool for laboratory diagnosis of porcine viral infections. However, interpreting the results requires considering the species and tissue origin of cell lines as well as the specific virus replication characteristics ...
Danila Moiseenko, Roman Chernyshev, Natalya Kamalova, Vera Gavrilova, Alexey Igolkin   +4 more
doaj   +1 more source

Multiplex nested RT-PCR for the detection of porcine enteric viruses

Journal of Virological Methods, 2010
Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine group A rotavirus (PRV-A) are major viruses causing enteric diseases of piglets. A multiplex nested reverse transcription polymerase chain reaction (multiplex nested RT-PCR) was developed for the detection of these viruses in field samples from piglets with ...
Salem, Abid Nabil Ben, Chupin Sergei, A., Bjadovskaya Olga, P., Andreeva Olga, G., Mahjoub, Aouni, Prokhvatilova Larissa, B.   +5 more
openaire   +2 more sources