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Purification and electron microscopy of potato leafroll virus
Virology, 1969A procedure for purification of potato leafroll virus (PLRV) from its plant host was improved. An extract from diseased Physalis floridana plants was emulsified with an n-butanol-chloroform mixture. The virus in the aqueous phase was concentrated by centrifugation, the pellet was resuspended in 0.01 M phosphate buffer and subjected to emulsification ...
M, Kojima +3 more
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Volunteer potatoes as a source of potato leafroll virus and potato virus X
American Potato Journal, 1981Volunteer potatoes were investigated as infection sources for potato leafroll virus (PLRV) and potato virus X (PVX) in a high elevation seed potato growing area of eastern Idaho. Population densities ofMyzus persicae were assessed. Percentage of PLRV and PVX infection of the volunteers and seed potato crops was determined, as well as density of ...
Garrett C. Wright, Guy W. Bishop
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Resistance to the Potato Leafroll Virus (PLRV) in Diploid Potatoes
Plant Breeding, 1989AbstractA high level of PLRV resistance has been found in four diploid genotypes originating from resistant ancestors widely utilized in European potato breeding. Plants of these genotypes were difficult to infect not only with aphids, but also with graft inoculation.
K. M. Swiezynski +2 more
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Characterization of potato leafroll virus
1980Potato leafroll virus (PLRV) was purified from infected potato (Splanum tuberosum L.) by using a mixture of chloroform and butanol for clarification and polyethylene glycol for concentration. The virus sedimented as a single band in a sucrose density gradient. Yield of purified virus varied from 0.4-0.6 mg/kg fresh weight of potato foliage. Virus yield
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Potato leafroll virus contains a double-stranded DNA
Virology, 1976Abstract The nucleic acid of potato leafroll virus (PLRV) has been isolated from highly purified virus preparations by two methods: (1) From a treatment with 0.5 N perchloric acid at 70° and quantitative estimation of the products, the DNA:protein ratio has been found to be nearly 40:60 (w/w).
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Chimeric Virus as a Source of the Potato Leafroll Virus Antigen
Molecular Biotechnology, 2017Large quantities of potato leafroll virus (PLRV) antigen are difficult to obtain because this virus accumulates in plants at a low titer. To overcome this problem, we constructed a binary vector containing chimeric cDNA, in which the coat protein (CP) gene of the crucifer infecting tobacco mosaic virus (crTMV) was substituted for the coat protein gene ...
Eugene V, Skurat +6 more
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Reaction to the potato leafroll virus (PLRV) in diploid potatoes
Potato Research, 1988Diploid parents with some resistance to PLRV, were intercrossed to give 3 families with 191 clones which were evaluated for reaction to PLRV and yielding ability. After inoculation with PLRV the clones could be separated into those: 1) resistant, 2) susceptible, 3) intolerant, reacting with low virus concentration, 4) tolerant and 5) intermediate in ...
K. M. Swiezyński +2 more
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Restricted multiplication of potato leafroll virus in resistant potato genotypes
Annals of Applied Biology, 1985SUMMARYPlants of a range of potato genotypes differing in rating for field resistance to potato leafroll virus (PLRV) were inoculated with the virus by grafting or by aphids (Myzus persicae). Plants of all genotypes tested became infected by each inoculation method and PLRV was detected by ELISA in the upper leaves of all genotypes within 26 days after
H. BARKER, B. D. HARRISON
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Increasing In-Row Spacing Enhances Potato Virus Y and Potato Leafroll Virus Spread in Potato
American Journal of Potato Research, 2015For potato, variety, tuber size, and chip or fresh markets all play a role in deciding which in-row spacing is used. Aphids are attracted to wide row spacings and as plant densities decrease, aphid densities increase. Wide in-row spacing could render ineffective any pest management tactic that exploited aphid colonization behavior through habitat ...
Jeffrey A. Davis +3 more
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Environmental factors influencing aphid transmission of potato virus Y and potato leafroll virus
Potato Research, 1988The effect of temperature, relative humidity (RH) and light on aphid transmission of potato virus Y (PVY) and potato leafroll virus (PLRV) was studied using as vectorsMyzus persicae Sulz. andAphis gossypii Glov. Host susceptibility was enhanced by 48 h pre-inoculation exposure at 25°C and by 48 h post-inoculation exposure to 30°C. High RH (80%) in both
M. N. Singh +3 more
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