Results 91 to 100 of about 6,587,859 (292)

Experimental and Theoretical Performance Analysis of a Building-Integrated Photovoltaic and Thermal (BIPVT) System [PDF]

open access: yesE3S Web of Conferences
Building integrated photovoltaic thermal (BIPVT) systems can generate electricity and heat from building envelope areas where deploying a photovoltaic thermal (PVT) system is challenging, as this study's experimental approach investigates and validates ...
Lee Heon-Min   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Multi-Source Data Fusion and Multi-Task Physics-Informed Transformer for Power Transformer Fault Diagnosis

open access: yesEnergies
Power transformers are critical assets in power systems, and their reliable operation is essential for grid stability. Conventional fault diagnosis methods suffer from delayed response and limited adaptability, while existing artificial intelligence ...
Yuanfang Huang   +2 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

open access: yesFEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer   +7 more
wiley   +1 more source

Graph Distance and Adaptive K-Nearest Neighbors Selection-Based Density Peak Clustering

open access: yesIEEE Access
Density Peak Clustering (DPC) is known for its rapid identification of cluster centers and successful clustering tasks. However, traditional DPC encounters several issues, which include simplifications in local density and distance metrics, a non-robust ...
Yuqin Sun   +4 more
doaj   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

open access: yesFEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata   +3 more
wiley   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

open access: yesAging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Formation of technical competencies in future electrical power engineers through professionally oriented physics education: a practice-based approach

open access: yesFrontiers in Education
This study examines the development of technical competencies among future electrical power engineers through professionally oriented physics education. A quasi-experimental pre-test–post-test design with experimental and control groups was used.
Gulnara Nauryzbayeva, Gulmira Gabdullina
doaj   +1 more source

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