Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Assessment of apparent nonstationarity in time series of annual inflow, daily precipitation, and atmospheric circulation indices: A case study from southwest Western Australia [PDF]
The southwest region of Western Australia has experienced a sustained sequence of low annual inflows to major water supply dams over the past 30 years. Until recently, the dominant interpretation of this phenomenon has been predicated on the existence of
Bates, BC +3 more
core +1 more source
Carbon Bond Mechanism IV (CBM-IV) is a widely used reaction mechanism in which VOCs are grouped according to the molecular structure. In the present study, we applied a sensitivity analysis on the CBM-IV mechanism to clarify the importance of each ...
Le Cao +3 more
doaj +1 more source
Derivation of the reduced reaction mechanisms of ozone depletion events in the Arctic spring by using concentration sensitivity analysis and principal component analysis [PDF]
The ozone depletion events (ODEs) in the springtime Arctic have been investigated since the 1980s. It is found that the depletion of ozone is highly associated with an auto-catalytic reaction cycle, which involves mostly the bromine-containing ...
L. Cao +4 more
doaj +1 more source
1 km monthly temperature and precipitation dataset for China from 1901 to 2017
. High-spatial-resolution and long-term climate data are highly desirable for understanding climate-related natural processes. China covers a large area with a low density of weather stations in some (e.g., mountainous) regions.
Shouzhang Peng +3 more
semanticscholar +1 more source
Microfluidic electro‐viscoelastic manipulation of extracellular vesicles
The electro‐viscoelastic manipulation as a potential method for separation of particles based on size. The particles introduced as a sheath flow migrate to the channel center under the influence of simultaneously applied electric field and pressure driven flow.
Seyedamirhosein Abdorahimzadeh +7 more
wiley +1 more source
The Hydrological Cycle over a Wide Range of Climates Simulated with an Idealized GCM [PDF]
A wide range of hydrological cycles and general circulations was simulated with an idealized general circulation model (GCM) by varying the optical thickness of the longwave absorber.
O'Gorman, Paul A., Schneider, Tapio
core +1 more source
The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity
We identified RNF219 as a CCR4‐NOT complex‐interacting E3 ubiquitin ligase that targets the CCR4‐NOT subunit CNOT6L for ubiquitination. RNF219 directly binds to the DUF3819 domain of CNOT1 through its putative α‐helix spanning amino acids 521–542. Our findings also suggest that antiproliferative activity of RNF219 is at least partially mediated by ...
Shou Soeda +9 more
wiley +1 more source
Aircraft Observations of Ice Particle Properties in Stratiform Precipitating Clouds
This study presented airborne measurements of ice particle properties in three stratiform precipitating clouds over northern China. By using horizontal observations at selected altitudes, the distributions of ice water content (IWC), particle habits, and
Tuanjie Hou +3 more
doaj +1 more source
TTT and R2TP chaperone complexes are required for the assembly and activation of mTORC1. WAC directly interacts with components of TTT, R2TP, and mTORC1, and these interactions are affected by the availability of glucose and glutamine, correlating with changes in mTORC1 activity.
Sofía Cabezudo +11 more
wiley +1 more source

