Results 161 to 170 of about 1,922,046 (318)

Editorial: Advancements in head and neck tumor treatment strategy driven by the perspective of precision oncology. [PDF]

Front Genet
Liao L, Feng Q, Xie S.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Advancing clinical precision medicine via peripheral blood immune single-cell omics. [PDF]

Clin Transl Med
Li L, Hu J, Duan W, Xu YM, Zhang J, Wang X.   +5 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Beyond Area Under the Receiver Operating Characteristic Curve: Evaluating Predictive Performance Metrics Under Class Imbalance in Real-World Clinical Data. [PDF]

JMIR Form Res
Ventura VDGJ, Andrade CMV, Almeida JM, Pessoa BP, Polanczyk CA, Nascimento GFD, Boersma E, Vianna HR, Farah KP, Rocha LCDD, Gonçalves MA, Marcolino MS.   +11 more
europepmc   +1 more source

Precision Chemistry in Precision Diagnostics

Precision Chemistry
Wenwan Zhong, Yang Liu, Rong Fan
openaire   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

PRECISION AGRICULTURE, WHOLE FIELD FARMING AND IRRIGATION PRACTICES: A FINANCIAL RISK ANALYSIS

One of the main advantages of precision agriculture (PA) is its potential to increase profitability by optimizing the productivity of each section of the field. Incorporating irrigation practices to the PA technology could further increase profitability.
Dillon, Carl R., Gandonou, Jean-Marc
core  

When Science Outpaces Infrastructure: People, Process, and Technology Challenges for Precision Dosing in Children. [PDF]

Clin Transl Sci
Lewis T, Jessa K, Ivaturi V, Noack A, Aluthge DP, Müller-Widmann D, Capello K, Fong M, Miatello A, Barwick M.   +9 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source