Results 111 to 120 of about 648,115 (257)

Dynamic changes of RNA-sequencing expression for precision medicine: N-of-1-pathways Mahalanobis distance within pathways of single subjects predicts breast cancer survival

, 2016
Poster exhibited at GPSC Student Showcase, February 24th, 2016, University of Arizona.Motivation: The conventional approach to personalized medicine relies on molecular data analytics across multiple patients.
Li, Haiquan, Lussier, Yves A., Achour, Ikbel, Li, Qike, Piegorsch, Walter W., Schissler, Grant A., Gardeux, Vincent   +6 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Identifying gene expression signatures for risk stratification of postoperative adjuvant chemotherapy in colorectal cancer

FEBS Open Bio, EarlyView.
A novel signature integrating genome‐wide analysis with clinical factors predicts recurrence in stage II colorectal cancer and enables a new risk stratification to guide postoperative adjuvant chemotherapy. Clinical risk stratification for postoperative recurrence in patients with pathological stage II (pStage II) colorectal cancer (CRC) is essential ...
Mayuko Otomo, Keisuke Okuno, Shuichi Watanabe, Sakiko Oba, Hiroyasu Kagawa, Masanori Tokunaga, Daisuke Ban, Yusuke Kinugasa   +7 more
wiley   +1 more source

Precision medicine in the clouds [PDF]

Nature Biotechnology, 2018
Vogt, Henrik, Green, Sara, Brodersen, John   +2 more
openaire   +2 more sources

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Bioanalysis for Precision Medicine [PDF]

Bioanalysis, 2019
Shuang, Yang, Zeren, Yang, Perry G, Wang
openaire   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Reporting guidelines for precision medicine research of clinical relevance : the BePRECISE checklist

Precision medicine should aspire to reduce error and improve accuracy in medical and health recommendations by comparison with contemporary practice, while maintaining safety and cost-effectiveness.
Rich, Stephen S, Lim, Siew S, Gomez, Maria F., Dennis, JM, Boyle, Jacqueline, Merino, J, Semnani-Azad, Zhila, Hivert, M-F, Sattar, N, Trenell, Michael I,, Boyle, Jacqueline,, Collin, C, Franks, Paul W,, Morieri, Mario L, Semnani-Azad, Z, Franks, Paul W., Sattar, Naveed,, Collin, Christian, Sargent, JL, Trenell, Michael I., Ahmad, Abrar,, Tobias, DK, Ramsay, M, Sanyal, AJ, Rich, SS, Lim, Siew S,, Ahmad, Abrar, Lim, Siew S., Trenell, MI, Hivert, Marie-France,, Ng, AH, Gomez, MF, Loos, RJF, Morrison, M, Dennis, John M, Gomez, Maria F, Sargent, Jennifer L, Langenberg, C, Ng, Ashley H, Trenell, Michael I, Lund University., Ahmad, A, Fitipaldi, Hugo, Morrison, Melinda,, Merino, Jordi,, Langenberg, Claudia,, Sargent, Jennifer L., Collin, Christian,, Hivert, Marie-France, Semnani-Azad, Zhila,, Ng, Ashley H., Loos, Ruth J F, Ng, Ashley H,, Morrison, Melinda, Langenberg, Claudia, Morieri, Mario L., Fitipaldi, Hugo,, Morieri, Mario L,, Franks, Paul W, Sanyal, Arun J,, Lim, SS, Sanyal, Arun J., Tobias, Deirdre K,, Tobias, Deirdre K., Ramsay, Michele, Tobias, Deirdre K, Loos, Ruth J F,, Morieri, ML, Sattar, Naveed, Hivert, Marie France, Boyle, J, Ramsay, Michele,, Franks, PW, Rich, Stephen S., Dennis, John M,, Dennis, John M., Rich, Stephen S,, Loos, Ruth J.F., Merino, Jordi, Fitipaldi, H, Sanyal, Arun J, Gomez, Maria F,, Sargent, Jennifer L,   +82 more
core   +1 more source