Results 41 to 50 of about 31,646 (297)

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

A novel method of prediction and optimization for preparative high-performance liquid chromatography separation

, 2008
The preparation of components from a complex sample is a difficult task. The optimization of the separation and subsequent scale-up is usually carried out by trial and error.
Xue, Xingya, Zhang, Jing, Jin, Yu, Xiao, Yuansheng, Shi, Hui, Liang, Xinmiao, 梁鑫淼, Liu, Yanfang, Zhang, Feifang   +8 more
core   +1 more source

Bioactivity-Guided High Performance Counter-Current Chromatography and Following Semi-Preparative Liquid Chromatography Method for Rapid Isolation of Anti-Inflammatory Lignins from Dai Medicinal Plant, Zanthoxylum acanthopodium var. timbor

Molecules, 2023
The development of Dai medicine is relatively slow, and Zanthoxylum has great economic and medicinal value. It is still difficult to obtain medicinal components from the low-polarity parts of Zanthoxylum belonging to Dai medicine.
Qing-Fei Fan, Lan Zhou, Pian-Chou Gongpan, Chuan-Li Lu, Hua Chang, Xun Xiang   +5 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Simultaneous detoxification and preparative separation of chlorogenic acid from Eupatorium adenophorum by combined column chromatography

, 2017
An efficient method for simultaneous detoxification and preparative separation of chlorogenic acid from Eupatorium adenophorum was developed. The entire procedure consisted of two separation steps.
Guo, Yuanheng, Liu, Boyan, Dong, Beitao, Zhang, Lijun, Zhao, Bing, Yuan, Xiaofan   +5 more
core   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Multi angle light scattering as a process analytical technology tool for real-time monitoring of molar mass of protein-polysaccharide conjugate fractions

Journal of Chromatography Open, 2022
Current polysaccharide conjugate vaccine manufacturing process involves conjugation of polysaccharide with protein followed by separation/purification of the conjugate from reaction components and free polysaccharide/protein, by preparative Size ...
Burki Rajendar, MVN Janardhan Reddy, Adusumilli Madhavi, Gullapalli Shravankumar, Ganti Sreenivasa Rao, Ramesh V. Matur   +5 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

A cyanopropyl reversed phase chromatography enables resolution of huperzine A and B on analytical and preparative scales

, 2005
The definitive version is available from www.wiley.comHuperzine A (HupA) and huperzine B (HupB) are two medically important components of Huperzia serrata.
Bi, J., Su, ZG, Zhang, GF, Sun, H., Li, X., Li, XN, Su, Z., Sun, HH, Bi, JX, Ma, R., Ma, GH, Zhang, G., Ma, G., Ma, RY   +13 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source