Results 81 to 90 of about 75,944 (308)

Separation and Purification of Zizyphusine, Spinosin, and 6′′′-Feruloylspinosin From

open access: yesNatural Product Communications, 2020
The methods of extraction, separation, and purification of zizyphusine, spinosin, and 6′′′-feruloylspinosin from Zizyphi Spinosi Semen ( ZSS ) were investigated.
Jiong Ran Wang   +3 more
doaj   +1 more source

Minimal Information About Sample Preparation for Phosphoproteomics [PDF]

open access: yes, 2009
This guideline describes parameters and conditions involved in phosphopeptide sample preparation. It covers from the description and preparation of the cells and tissues to the fractionation and specific enrichment of phosphopeptides for MS analysis. The
David Ovelleiro   +3 more
core   +2 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

open access: yesFEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel   +13 more
wiley   +1 more source

Heterocyclic organosilicon compounds separation by preparative gas chromatography

open access: yesТонкие химические технологии, 2008
The preparative gas chromatography method has been used for isolation of heterocyclic organosilicon compounds boiling up to 400o C. The ways are shown for solution of problems which arise in the course of isolation of such high boiling substances.
G. N. Turkeltaub   +2 more
doaj  

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

open access: yesFEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa   +3 more
wiley   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

open access: yesFEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga   +3 more
wiley   +1 more source

STRUCTURE OF METHYLPHEOPHORBIDE-RCI [PDF]

open access: yes, 1986
he methanolic extract of the cyanobacterium (blue-green alga) Spirulina geitleri has been treated with methanolic acid to convert all chlorophyllous pigments to their methylpheophorbides.
Budzikiewicz H.   +27 more
core   +1 more source

AAA+ protein unfoldases—the Moirai of the proteome

open access: yesFEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Supercritical fluid chromatography and scale up study

open access: yesActa Chimica Slovenica, 2014
The influence of process parameters on supercritical fluid chromatography (SFC) and scale-up on to the preparative scale was investigated. In this scope, dependency of pressure, temperature, type and concentration of modifier, and type of stationary ...
Miha Oman   +3 more
doaj  

Enantiomeric Ratio Changes of Terpenes in Essential Oils from Hybrid Eucalyptus grandis × E. tereticornis and its Pure Species [PDF]

open access: yes, 2018
Some Eucalyptus species produce oils with biological activities and the effect of their interspecifc hybridization on the enantiomeric composition of terpenes has not been reported.
Alvarez Costa, Agustin   +4 more
core   +2 more sources

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