Results 151 to 160 of about 5,609,198 (311)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

NEW PROTOCOL FOR IAD TREATMENT IN PEDIATRIC PATIENTS: STABLE OZONIDES

open access: yes
NEW PROTOCOL FOR IAD TREATMENT IN PEDIATRIC PATIENTS: STABLE OZONIDES Serena Crucianelli1 , Guido Ciprandi2 , Mauro Pace3 , Valentina Vanzi4 , Monica Rossi5 , Giancarlo Antonielli6 1 Sapienza University of Rome, Internal Medicine and Medical ...
SERENA CRUCIANELLI   +5 more
core  

Pressure-relief joint sample map.

open access: yes
To address the problem of large deformations in weak surrounding rock tunnels under high ground stress, which cause damage to initial support structures, this study proposes a novel type of circumferential pressure-relief joint based on the concept of ...
Zhiming Xu (1271712)   +1 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Clinical use of ambulatory blood pressure monitoring.

open access: yes, 1999
Beilin, L   +4 more
core   +1 more source

Home - About - Disclaimer - Privacy