Results 71 to 80 of about 4,566,256 (295)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

A retrospective study of sodium hypochlorite pulpotomies in primary molars

Journal of Dental Sciences, 2016
Background/purpose: Formocresol has been a popular pulpotomy medicament for primary molars, however, its toxicity and potential carcinogenicity leaves room for other alternatives such as sodium hypochlorite (NaOCl).
Tzu-Ying Li, Li-Chuan Chuang, Aileen I. Tsai   +2 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Analytical evidence of enamel hypomineralisation on permanent and primary molars amongst past populations [PDF]

, 2017
Molar Incisor Hypomineralisation (MIH) and Hypomineralised Second Primary Molars (HSPM) involve prevalent qualitative structural developmental anomalies of tooth enamel affecting the first permanent molars (and often incisors) and the second primary ...
Manton, D, Christine Couture-Veschambre, Patrick Rouas, Elsa Garot, Rouas, P, David Manton, Couture-Veschambre, C, Beauval, C, Garot, E, Cédric Beauval   +9 more
core   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Evaluation of local anaesthesia techniques preferred by paediatric dentists in pulpal procedures for mandibular primary molars. [PDF]

BMC Oral Health
Aim This study aimed to evaluate the local anaesthesia methods preferred by paediatric dentists for pulpal treatments of mandibular primary molars, along with alternative techniques used when the initial approach failed. Method A 23-item web-based survey
Senem Özsunkar P, Duman S.
europepmc   +2 more sources

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Root Resorption Patterns of Primary Mandibular Molars and Location of Premolar Successors Through Radiographs in Pakistani Population

Pakistan Journal of Medicine and Dentistry
Background: Primary teeth undergo physiological root resorption followed by eruption of their permanent successors. Roots of primary mandibular molars can have either symmetrical or asymmetrical resorption patterns.
Sadaf Ali, Tasleem Hosein, Khizra Rehman, Rafia Ruaaz, Yasir Raza, Pooja Balani   +5 more
doaj   +1 more source

Are Hypomineralized Primary Molars and Canines Associated with Molar-Incisor Hypomineralization?

Pediatric dentistry, 2018
The purpose of this study was to evaluate the prevalence of and relationship between hypomineralized second primary molars (HSPM) and hypomineralized primary canines (HPC) with molar-incisor hypomineralization (MIH) in 1,963 schoolchildren.The European Academy of Paediatric Dentistry (EAPD) criterion was used for scoring HSPM/HPC and MIH. Only children
Da Silva Figueiredo Sé, Maria Jose, Ribeiro, Ana Paula Dias, Dos Santos-Pinto, Lourdes Aparecida Martins, De Cassia Loiola Cordeiro, Rita, Cabral, Renata Nunes, Leal, Soraya Coelho   +5 more
openaire   +2 more sources