Results 161 to 170 of about 93,364 (279)

Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells. [PDF]

STAR Protoc
Wu Y, Sidharta M, Zhong A, Persily B, Li M, Zhou T.   +5 more
europepmc   +1 more source

CRISPR/Cas9 or prime editing? – It depends on… [PDF]

Open Life Sciences, 2020
openaire   +3 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Implications of the FDA's new plausible mechanism framework for the development of a personalized in vivo prime editing platform. [PDF]

Am J Hum Genet
Feierman ER, Whittaker MN, Quigley A, Brooks DL, McVeigh P, Nan AX, Hsu A, Said H, Soliman OY, Giovenco R, Davidson BL, Alameh MG, Liu DR, Wang X, Musunuru K, Ahrens-Nicklas RC.   +15 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Development of an ultra-efficient prime editing system in tomato. [PDF]

Nat Commun
Van Vu T, Thi Nguyen N, Kim J, Hoai Nguyen T, Kim JY.   +4 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Prime editing of the common Familial Dysautonomia-causing c.2204 + 6T > C splicing mutation. [PDF]

Orphanet J Rare Dis
Peretto L, Pinotti M, Balestra D.
europepmc   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

A multiplex, prime editing framework for identifying drug resistance variants at scale. [PDF]

Cell Genom
Abadie FMC, Suiter CC, Smith NT, Daza RM, Rominger MC, Parrish P, McDiarmid TA, Lalanne JB, Martin B, Calderon D, Ellison A, Berger AH, Shendure J, Starita LM.   +13 more
europepmc   +1 more source