Results 61 to 70 of about 2,833 (262)

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

open access: yesFEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

open access: yesFEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose   +9 more
wiley   +1 more source

Generalized roughness in (∈,∈∨q)-fuzzy ideals of hemirings

open access: yesKuwait Journal of Science, 2017
Generalized roughness for fuzzy ideals in hemirings is studied. Approximations for fuzzy prime ideals are discussed. It is shown that generalized lower approximation as well as generalized upper approximation of (∈ , ∈∨q)-fuzzy prime (semiprime) ideals ...
Muhammad Rameez   +2 more
doaj  

Prime and primary ideals in semirings

open access: yesOsaka Journal of Mathematics, 2015
We study zero divisors and minimal prime ideals in semirings of characteristic one. Thereafter we find a counterexample to the most obvious version of primary decomposition, but are able to establish a weaker version. Lastly, we study Evans'condition in this context.
openaire   +5 more sources

Prime Ideals in Matrix Rings [PDF]

open access: yesProceedings of the Glasgow Mathematical Association, 1956
Let R be a ring and let Rn be the complete ring of n × n matrices with coefficients from R.
openaire   +2 more sources

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

open access: yesFEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson   +2 more
wiley   +1 more source

ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function

open access: yesFEBS Open Bio, EarlyView.
Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley   +5 more
wiley   +1 more source

On Hadamard algebras

open access: yesLe Matematiche, 2000
Topological algebras of sequences of complex numbers are introduced, endowed with a Hadamard product type. The complex homomorphisms on these algebras are characterized, and units, prime cyclic ideals, prime closed ideals, and prime minimal ideals ...
Carlos C. Peña
doaj  

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

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