Results 61 to 70 of about 2,833 (262)
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Generalized roughness in (∈,∈∨q)-fuzzy ideals of hemirings
Generalized roughness for fuzzy ideals in hemirings is studied. Approximations for fuzzy prime ideals are discussed. It is shown that generalized lower approximation as well as generalized upper approximation of (∈ , ∈∨q)-fuzzy prime (semiprime) ideals ...
Muhammad Rameez +2 more
doaj
Prime and primary ideals in semirings
We study zero divisors and minimal prime ideals in semirings of characteristic one. Thereafter we find a counterexample to the most obvious version of primary decomposition, but are able to establish a weaker version. Lastly, we study Evans'condition in this context.
openaire +5 more sources
Prime Ideals in Matrix Rings [PDF]
Let R be a ring and let Rn be the complete ring of n × n matrices with coefficients from R.
openaire +2 more sources
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function
Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley +5 more
wiley +1 more source
Topological algebras of sequences of complex numbers are introduced, endowed with a Hadamard product type. The complex homomorphisms on these algebras are characterized, and units, prime cyclic ideals, prime closed ideals, and prime minimal ideals ...
Carlos C. Peña
doaj
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source

