Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Complete genome sequences of soil-associated <i>Paenibacillus polymyxa</i> strains ALE1, ALE2, and ALE3 generated by PacBio sequencing. [PDF]
Tran TD, Lee S, Hnasko R, McGarvey JA.
europepmc +1 more source
ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function
Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley +5 more
wiley +1 more source
Subunit vaccination using Atomic Layering Thermostable Antigen and Adjuvant (ALTA<sup>®</sup>) platform elicits enhanced humoral and cellular immune responses. [PDF]
Ivanova DL +12 more
europepmc +1 more source
Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito +7 more
wiley +1 more source
Heterologous saRNA prime - multivalent protein boost strategy induces broad and durable immunity against SARS-CoV-2 and MERS-CoV. [PDF]
Renn D +8 more
europepmc +1 more source
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
CRISPR-associated transposon for programmable viral vector engineering and prime editing. [PDF]
Dang QT +6 more
europepmc +1 more source
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
Heterologous Prime-Boost Vaccination with GRA35-Encoding DNA and mRNA Vaccines Enhances Protective Immunity Against <i>Toxoplasma gondii</i> Infection in Mouse Models. [PDF]
Chen S, Li R, Zhu Y, Sun J, Chen J.
europepmc +1 more source

