Results 241 to 250 of about 18,644,533 (316)

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

Enhancing Clinical Trial Sites in Low- and Middle-Income Countries to Facilitate Product Development in Response to the COVID-19 Pandemic. [PDF]

Clin Infect Dis
Kang SSY, Tadesse BT, Jeon HJ, Fallah MP, Dereje N, Ilesanmi OS, Abay SM, Tekle BI, Son HG, Shrestha L, Shrestha A, Ramiso HV, Canouet V, Kim Y, Asante KP, Anim J, Kaali S, Capeding MR, Jani IV, Capitine I, Mahumane A, Wartel TA, Haselbeck AH, Saluja T, Marks F.   +24 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Integrate & balance aspects for safe and sustainable innovation: Needs analysis on SSbD categories and product development stage requirements to cover the entire life cycle. [PDF]

Comput Struct Biotechnol J
Larrea-Gallegos GM, Hofer S, Hofstätter N, Punz B, Watzek N, Lölsberg W, Wiench K, Wohlleben W, Aguirre IG, Athanassios N, Sarimveis H, Costa A, Seitz C, Friedrichs S, Exner TE, Hischier R, Marvuglia A, Himly M.   +17 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

QbD product development: rapid optimization and scale-up of PBAE-based siRNA delivery <i>via</i> DoE-guided microfluidics.

RSC Pharm
Kromer APE, Eller LJM, Jürgens DC, Merkel OM.   +3 more
europepmc   +1 more source

Gender integration of agricultural innovation: implications for the genetically modified crop product development pipeline. [PDF]

GM Crops Food
Katz E.
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

The Platform Technology Approach to mRNA Product Development and Regulation. [PDF]

Vaccines (Basel)
Skerritt JH, Tucek-Szabo C, Sutton B, Nolan T.   +3 more
europepmc   +1 more source