Results 61 to 70 of about 774,784 (264)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Resultado econômico da produção de ovinos para carne em pasto de azevém e confinamento = Economic results of sheep production on ryegrass pasture or feedlot

Acta Scientiarum: Animal Sciences, 2009
O trabalho objetivou analisar a viabilidade econômica da produção de cordeiros para carne e identificar os componentes que exercem maior influência sobre o custo de produção. Este estudo foi realizado em duas etapas. Primeira etapa: experimento no Lapoc-
Carina Simionato de Barros, Alda Lúcia Gomes Monteiro, César Henrique Espírito Candal Poli, Maria Angela Machado Fernandes, Rodrigo de Almeida, Sergio Rodrigo Fernandes   +5 more
doaj  

Economic benefit of an optimized copper spray program for citrus canker and black spot control in Brazil [PDF]

Scientia Agricola
Copper-based formulations are used extensively to manage two of the leading citrus diseases that affect the São Paulo (SP) citrus belt, Brazil, namely, citrus canker and citrus black spot.
Franklin Behlau, Geraldo José Silva-Junior, Rafaele Regina Moreira, Eduardo Cassettari Monteferrante, Andreia Cristina de Oliveira Adami, Sílvia Helena Galvão de Miranda   +5 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

Development of a Swine Production Cost Calculation Model. [PDF]

Animals (Basel), 2022
Alves LKS, Gameiro AH, Schinckel AP, Garbossa CAP.   +3 more
europepmc   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Assessing the operational efficiency of the agricultural machinery fleet at a modern agro-industrial enterprise

Управление
In the cost structure of agricultural production, fuel and lubricant costs (hereinafter referred to as FLC) account for a significant share and are subject to high price volatility, which creates substantial economic risks.
M. Yu. Karelina, M. S. Chekusov, D. V. Serdechnyi, V. V. Filatov, V. D. Kutkov, N. S. Akinshin   +5 more
doaj   +1 more source