Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Use of microsoft project program in production process organization
, 2018 V. V. Kruglikova, T. O. Paromova
openalex +2 more sources
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Correction to "DNA Nanotags for Multiplexed Single-Particle Electron Microscopy and <i>In Situ</i> Electron Cryotomography". [PDF]
JACS AuChen Y, Huang Y, Yang YR.
europepmc +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
FEBS Open Bio, EarlyView.Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Improving nitrogen management in US agriculture: Yield Reserve versus land retirement. [PDF]
J Environ QualHu C, Zhang W, Bosch D.
europepmc +1 more source
CC336 1987 Production Adjustment Program (Worksheets for Feed Grains and Wheat Producers)
, 2014 A. L. Frederick, Larry L. Bitney
openalex +1 more source
Evaluation of Crestar® and modified Crestar programs for timed insemination in lactating Egyptian buffaloes (Bubalus bubalis) under intensive production system [PDF]
, 2008 Mortada M.A. Hussein +2 more
openalex +1 more source