Results 81 to 90 of about 1,871 (258)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Biogeocenosis development during initial revegetation of a coal combustion ash dump

BIO Web of Conferences, 2018
The initial stage of biogeocenoses development on the coal ash dump produced by the thermal power staton in Novosibirsk (55.000, 83.068), Russia, were studied after 9 years of spontaneous revegetation.
Sheremet Natalia, Belanov Ivan, Doronkin Vladimir, Lamanova Tatiana, Naumova Natalia   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Mumford coverings of the projective line

Archiv der Mathematik, 2003
In a famous paper of 1987 [in: Number theory, Semin. New York 1984/85, Lect. Notes Math. 1240, 165--195 (1987; Zbl 0627.12015)], \textit{D. Harbater} proved that any finite group \(G\) can be realized as the Galois group of a finite normal covering of the projective line over a given complete non-Archimedean field \(K\).
Put, Marius van der, Voskuil, Harm H.
openaire   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Grass cover and meadow forage reservein the Cervus elaphus L. breeding

Наукові праці Лісівничої академії наук України, 2019
Species composition, projective cover, frequency of occurrence, abundance and grass reserve on the out of agricultural use lands in conditions of the Western Polissia of Ukraine are characterized.
Vasyl Mazepa, Bohdan Kolisnyk, Pavlo Khoetskyi   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Projective structures inducing covering maps

Duke Mathematical Journal, 1995
The author considers parametrization of the projective structures on a compact Riemann surface \(S\) of genus \(\geq 2\). The pullback of a projective structure on \(S\) determines a projective structure on the universal covering space \(\widetilde U\) of \(S\), and the analytic continuation of the projective coordinate charts defines a local ...
openaire   +3 more sources