Results 61 to 70 of about 5,971 (229)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

The Projective Generalization of Certain Well-Known Curves

open access: yes, 1941
As expressed in the title, the purpose of this thesis is to projectively generalize certain well-known curves. These curves, which are the cissoid of diocles and the conchoid of Nicomedes, are ordinarily associated with metric geometry. Their equations
Jautz, Martin L.
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

open access: yesFEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao   +3 more
wiley   +1 more source

Cyclic projective planes and Wada dessins

open access: yes, 2001
Bipartite graphs occur in many parts of mathematics, and their embeddings into orientable compact surfaces are an old subject. A new interest comes from the fact that these embeddings give dessins d’enfants providing the surface with a unique structure ...
Streit, Manfred, Wolfart, Jürgen
core  

Simulating quantum annealing via projective quantum Monte Carlo algorithms [PDF]

open access: yes, 2018
In this thesis, we implement projective quantum Monte Carlo (PQMC) methods to simulate quantum annealing on classical computers. A detailed derivation of the algorithm is presented.
Inack, Estelle Maeva
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Experimental demonstration of continuous quantum error correction. [PDF]

open access: yesNat Commun, 2022
Livingston WP   +5 more
europepmc   +1 more source

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