Results 171 to 180 of about 12,746 (209)

Ubiquinone biosynthesis in Leishmania major promastigotes

International Journal for Parasitology, 1995
Promastigotes of Leishmania major contain a ubiquinone which has a side chain made up of nine isoprene subunits (UQ9). Incorporation of radioactivity from [14C] acetate and [14C] mevalonate into ubiquinone as well as the identification of hydroxymethylglutaryl coenzyme A reductase (HMG CoA reductase), and mevalonate kinase indicate that the isoprenoid ...
G, Ranganathan, A J, Mukkada
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Glucose transport in Leishmania donovani promastigotes

Molecular and Biochemical Parasitology, 1984
Mid-log phase Leishmania donovani promastigotes accumulated 2-deoxy-D-glucose (2-DOG) via a carrier mediated transport system, maintaining an apparent Km of 24.4 microM and a Vmax of 3.12 nmol mg-1 protein min-1. D-Glucose but not L-glucose competitively inhibited the 2-DOG transport with an apparent Ki of 18.7 microM.
D, Zilberstein, D M, Dwyer
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Leishmania donovani: Hemolytic Activity of Promastigotes

Experimental Parasitology, 1994
A hemolytically active component was found to be present in Leishmania donovani promastigotes for the first time. It lysed human and rabbit erythrocytes to varying degrees. The optimal pH for the activity was found to be 5.8. The rate of hemolysis was dependent on both erythrocyte and parasite concentrations.
R, Chakravarty, M C, Sharma, A K, Gupta, N, Prakash, R, Saran   +4 more
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A simple method for cloning leishmanial promastigotes

Zeitschrift f�r Parasitenkunde Parasitology Research, 1986
A method of cloning leishmanial promastigotes is described in which mid-exponential phase cultures are diluted to contain approximately 3 X 10(3) promastigotes per ml. Hanging-drop preparations made from 0.2-0.4 microliter volumes of the diluted culture seen to contain a single promastigote are picked-up in glass capillary tubes.
D A, Evans, V, Smith
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Putrescine transport system in Leishmania infantum promastigotes

Molecular and Biochemical Parasitology, 1989
A transport system for putrescine in Leishmania infantum (= L. donovani infantum) promastigotes has been identified and characterized by measuring the uptake of radioactively labelled putrescine. Putrescine uptake was time- and temperature-dependent without any accumulation taking place at 0 degrees C. Uptake of putrescine was maximal at pH values near
R, Balaña-Fouce, D, Ordóñez, J M, Alunda   +2 more
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Neutral amino acid transport in Leishmania promastigotes

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1983
Neutral amino acid transport was investigated in Leishmania promastigotes. Proline and alanine transport occur against their concentration gradient although there is a very rapid (40% at 30 min) conversion of proline to alanine. Uptake of these amino acids occurs by a sodium-independent route which is completely eliminated by addition of CCCP or KCN ...
P, Bonay, B E, Cohen
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Heme metabolism in promastigotes of Leishmania donovani

Molecular and Cellular Biochemistry, 1997
Promastigotes of Leishmania donovani (Dd-8 strain) showed presence of important key enzymes of heme synthesizing (delta-aminolevulinic acid synthase and ferrochelatase) and degrading (heme oxygenase and biliverdin reductase) systems, classical leishmanicidal drugs viz allopurinol, amphotericin B, pentamidine and CDRI compound 93/202 inhibited the heme ...
P, Srivastava, G D, Sharma, K K, Kamboj, A K, Rastogi, V C, Pandey   +4 more
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Eosinophil chemotactic activity in Leishmania amazonensis promastigotes

Parasitology Research, 1996
Tissue eosinophilia was observed in the subcutaneous tissue of mice shortly after their inoculation not only with living but also with lysed promastigotes of Leishmania amazonensis. Intraperitoneal inoculation of lysed promastigotes from five different Leishmania species (L. donovani, L. chagasi, L. tropica, L. amazonensis, and L. braziliensis) induced
S, Saito, A, Hamada, N, Watanabe, T, Obata, K, Katakura, H, Ohtomo   +5 more
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Toxicity of bilirubin to Leishmania tropica promastigotes

Experimental Parasitology, 1976
Abstract Treatment of Leishmania tropica promastigotes with bilirubin results in loss of viability and death of the cells. A concentration of 0.1 m M is sufficient to cause complete inhibition of growth. Bilirubin inhibits the uptake of 2-deoxy- d -glucose and l -methionine. It increases the efflux of intracellularly accumulated sugars (both free
M W, Simon, J M, Rusnak, A J, Mukkada
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The promastigote surface protease of leishmania

Parasitology Today, 1987
The infective forms o f several protozoan parasites are covered with a limited number o f major surface proteins. In this respect, Leishmania is no exception, and recent investigations have demonstrated on promostigotes the presence o f a single surface glycoprotein.
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