Results 1 to 10 of about 97,027 (246)

Thymus and Leishmania at the Crossroads: Autoimmunity and Cancer. [PDF]

Immunology
Conceptual ‘Crossroads Model’ linking Leishmania, thymic infection and immune dysregulation: an integrative model is proposed in which Leishmania infection disrupts thymic architecture and stromal–thymocyte interactions. These alterations converge to promote defective tolerance, increased autoimmune risk and a microenvironment permissive to malignant ...
Barros ABB, Leite GA, de Andrade AG, Reis MDDS, Cavalcante-Silva LHA, Lins MP.   +5 more
europepmc   +2 more sources

Antibiotic-Free Nanoplasmids as Promising Alternatives for Conventional DNA Vectors

Vaccines, 2022
DNA vaccines with their extraordinary properties are the best choice as vectors for subunit vaccines but are not in compliance with safety regulations, mainly because of the antibiotic resistance genes on their backbone.
Negar Seyed, Farnaz Zahedifard, Sima Habibzadeh, Roya Yousefi, Mahya Sadat Lajevardi, Elham Gholami, Sima Rafati   +6 more
doaj   +1 more source

Leishmania tarentolae as Potential Live Vaccine Co-Expressing Distinct Salivary Gland Proteins Against Experimental Cutaneous Leishmaniasis in BALB/c Mice Model

Frontiers in Immunology, 2022
Leishmaniasis is a neglected vector-borne disease caused by Leishmania parasites transmitted through the infected sand flies bite. Current treatments are limited, partly due to their high cost and significant adverse effects, and no human vaccine is yet ...
Mahya Sadat Lajevardi, Mahya Sadat Lajevardi, Elham Gholami, Tahereh Taheri, Hamzeh Sarvnaz, Sima Habibzadeh, Negar Seyed, Yousef Mortazavi, Sima Rafati   +8 more
doaj   +1 more source

Structural analysis of PpSP15 and PsSP9 sand fly salivary proteins designed with a self-cleavable linker as a live vaccine candidate against cutaneous leishmaniasis

Parasites & Vectors, 2022
Background Leishmania parasites are deposited in the host through sand fly bites along with sand fly saliva. Therefore, salivary proteins are promising vaccine candidates for controlling leishmaniasis.
Mahya Sadat Lajevardi, Tahereh Taheri, Elham Gholami, Negar Seyed, Sima Rafati   +4 more
doaj   +1 more source

Promastigote-to-Amastigote Conversion in Leishmania spp.—A Molecular View

Pathogens, 2022
A key factor in the successful infection of a mammalian host by Leishmania parasites is their conversion from extracellular motile promastigotes into intracellular amastigotes. We discuss the physical and chemical triggers that induce this conversion and
Joachim Clos, Janne Grünebast, Myrine Holm   +2 more
doaj   +1 more source

Cultivation of Protozoa Parasites In Vitro: Growth Potential in Conventional Culture Media versus RPMI-PY Medium

Veterinary Sciences, 2023
The in vitro cultivation of Leishmania and Trypanosoma parasites plays an important role in the diagnosis and treatment of parasitic diseases. Although Evans’s modified Tobie and Novy–MacNeal–Nicolle media, for Leishmania spp.
Germano Castelli, Eugenia Oliveri, Viviana Valenza, Susanna Giardina, Flavia Facciponte, Francesco La Russa, Fabrizio Vitale, Federica Bruno   +7 more
doaj   +1 more source

Experimental treatment with sodium stibogluconate of hamsters infected with Leishmania (Leishmania) chagasi and Leishmania (Leishmania) amazonensis [PDF]

Revista da Sociedade Brasileira de Medicina Tropical, 1999
The present paper reports the experimental treatment of hamsters infected with Leishmania chagasi and Leishmania amazonensis with sodium stibogluconate (20mg/kg/day x 20 days). Only with L. chagasi did the treatment result in the complete elimination of parasites from the spleen. However, no parasitological cure was achieved in hamsters infected with L.
Figueiredo, Elizabeth M. de, Silva, Jaime Costa e, Brazil, Reginaldo P.   +2 more
openaire   +4 more sources

Comparison of Protective Potency of DNA and Live Vaccines Expressing A2-CPA-CPB-CTE Antigens against Visceral Leish-maniasis in Syrian Hamster as Preliminary Study

Iranian Journal of Parasitology, 2020
Background: Visceral leishmaniasis is the most severe form of leishmaniasis caused by Leishmania (L.) donovani complex. Drug-resistant strains have been developed as a consequence of the current chemotherapeutic interventions, which has increased the ...
Yasaman TASLIMI, Farnaz ZAHEDIFARD, Tahereh TAHERI, Delaram DOROUD, Sakineh LATIF DIZAJI, Noushin SALJOUGHIAN, Sima RAFATI   +6 more
doaj   +1 more source

Isolation, characterization, and functional study of extracellular vesicles derived from Leishmania tarentolae

Frontiers in Cellular and Infection Microbiology, 2022
Leishmania (L.) species are protozoan parasites with a complex life cycle consisting of a number of developmental forms that alternate between the sand fly vector and their host. The non-pathogenic species L.
Mehrdad Shokouhy, Mehrdad Shokouhy, Hamzeh Sarvnaz, Yasaman Taslimi, Mahya Sadat Lajevardi, Sima Habibzadeh, Amir Mizbani, Faezeh Shekari, Mandana Behbahani, Ana Claudia Torrecilhas, Sima Rafati   +10 more
doaj   +1 more source

Molecular Diagnosis of Leishmaniasis: Quantification of Parasite Load by a Real-Time PCR Assay with High Sensitivity

Pathogens, 2021
Real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to achieve a sensitivity of 1 parasite/mL. For this purpose, we cloned the conserved kDNA fragment of 120 bp into competent cells and correlated them with serial ...
Germano Castelli, Federica Bruno, Stefano Reale, Simone Catanzaro, Viviana Valenza, Fabrizio Vitale   +5 more
doaj   +1 more source