Results 21 to 30 of about 57,558 (196)

Bulky pcDNA plasmid encoding Leishmania- and Sandfly-derived antigens resulted in transient Th1 response post Leishmania major infection in BALB/c mice

Scientific Reports
Leishmaniasis is a common parasitic infection in tropical regions worldwide, and yet there is no effective human vaccine available. The results of leishmanization in animal models have emphasized the role of antigen persistence after recovery from the ...
Negar Seyed, Hamzeh Sarvnaz, Sima Habibzadeh, Roya Yousefi, Zahra Eghbali, Elham Gholami, Tahereh Taheri, Sima Rafati   +7 more
doaj   +1 more source

Optimized Mouse BMDC Isolation and Culture under Endotoxin-Free Conditions

Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām, 2021
Introduction: Dendritic cells are very important in basic studies and vaccine research, but isolation and culture of these cells face challenges due to their small number in tissues.
Atefeh Sadeghi Shermeh, Sima HAbibzadeh, Adeleh Taghikhani, Sima Rafati, Negar Seyed   +4 more
doaj  

Leishmania kinetoplast DNA contributes to parasite burden in infected macrophages: Critical role of the cGAS-STING-TBK1 signaling pathway in macrophage parasitemia

Frontiers in Immunology, 2022
Leishmania parasites harbor a unique network of circular DNA known as kinetoplast DNA (kDNA). The role of kDNA in leishmania infections is poorly understood. Herein, we show that kDNA delivery to the cytosol of Leishmania major infected THP-1 macrophages
Ismail Cem Yilmaz, Ismail Cem Yilmaz, Emre Dunuroglu, Ihsan Cihan Ayanoglu, Emre Mert Ipekoglu, Muzaffer Yildirim, Nogay Girginkardesler, Yusuf Ozbel, Seray Toz, Ahmet Ozbilgin, Gamze Aykut, Ihsan Gursel, Ihsan Gursel, Mayda Gursel, Mayda Gursel   +14 more
doaj   +1 more source

Draft Genome Sequences of Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) mexicana, and Leishmania (Leishmania) aethiopica, Potential Etiological Agents of Diffuse Cutaneous Leishmaniasis.

Microbiology resource announcements, 2019
We present here the draft genome sequences of Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) mexicana, and Leishmania (Leishmania) aethiopica, potential etiological agents of diffuse cutaneous leishmaniasis (DCL). Sequence data were obtained using PacBio and MiSeq platforms.
Batra, Dhwani, Lin, Wuling, Narayanan, Vidhya, Rowe, Lori A., Sheth, Mili, Zheng, Yueli, Loparev, Vladimir, de Almeida, Marcos   +7 more
openaire   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Gardiquimod Nanoemulsion Targets Cutaneous Leishmaniasis Lesions Reducing Systemic Toxicity and Parasite Burden

Advanced Healthcare Materials, EarlyView.
Nanoemulsion delivery of a TLR7 agonist enhances safety and promotes colocalization with Leishmania‐infected macrophages in skin lesions, enabling a ∼2‐log reduction in parasite burden. However, tissue‐level constraints and TLR‐driven regulatory feedback generate a mixed immune response that limits complete parasite clearance.
Carmen Palomino‐Cano, M. Carmen Mera‐Delgado, Esther Moreno, Esther Larrea, Lecnia Aguirre, Jonathan Miguel Zanatta, Sónia Barros‐Carvalho, Iola F. Duarte, Ricardo Silvestre, Juan M. Irache, Javier Carrión, Socorro Espuelas   +11 more
wiley   +1 more source

Calpain Proteases and the Evolving Signaling Network in Insect Embryonic Patterning

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
Insect embryonic Dorsal‐Ventral (DV) patterning relies on the BMP and Toll pathways to different extents. Calcium‐dependent cystein proteases of the Calpain family also exert an important function to pattern the DV axis. In Drosophila, Calpain A cleaves the Cactus/IkappaB inhibitor and modifies Toll signals in ventral regions of the embryo. In Rhodnius
Alison Julio, Helena Araujo
wiley   +1 more source

Leishmania naiffi and Leishmania guyanensis reference genomes highlight genome structure and gene evolution in the Viannia subgenus [PDF]

Royal Society Open Science, 2018
The unicellular protozoan parasite Leishmania causes the neglected tropical disease leishmaniasis, affecting 12 million people in 98 countries. In South America, where the Viannia subgenus predominates, so far only L. (Viannia) braziliensis and L.
Simone Coughlan, Ali Shirley Taylor, Eoghan Feane, Mandy Sanders, Gabriele Schonian, James A. Cotton, Tim Downing   +6 more
doaj   +1 more source

Eugenol-Rich Essential Oil from Pimenta dioica: In Vitro and In Vivo Potentialities against Leishmania amazonensis

Pharmaceuticals, 2023
Pimenta dioica L. is one the most recognized species with diverse biological activities. In this study, in vitro activity and in vivo efficacy of essential oil from P. dioica (EO-Pd) was evaluated.
Lianet Monzote, Laura Machín, Adiel González, Ramón Scull, Yamilet I. Gutiérrez, Prabodh Satyal, Lars Gille, William N. Setzer   +7 more
doaj   +1 more source