FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Why Uncertainty Is Essential for Consciousness: Local Prospect Theory vs. Predictive Processing. [PDF]
Entropy (Basel)Heylighen F, Beigi S.
europepmc +1 more source
Four-Way Evolutionary Game Analysis of Government Project Bidding Collusion in a State of Limited Rationality Based on Prospect Theory. [PDF]
Comput Intell Neurosci, 2022 Ma C, Chen Y, Nie S.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Decision-making of construction workers' waste reduction behavior: a study based on Cost-Benefit Theory and Cumulative Prospect Theory. [PDF]
Front PsycholXu S +6 more
europepmc +1 more source
Operational efficiency evaluation of urban and rural residents' basic pension insurance system by utilizing a picture fuzzy TOPSIS method based on the cumulative prospect theory. [PDF]
Front Public Health, 2022 Xiong Y.
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Risk assessment of existing buildings in tunnel construction based on an improved cumulative prospect theory method. [PDF]
Sci ProgChang L, Nordin N, Gu X, Zhao Y.
europepmc +1 more source
An extended MABAC method based on prospect theory with unknown weight information under Fermatean fuzzy environment for risk investment assessment in B&R. [PDF]
J Ambient Intell Humaniz Comput, 2022 Tan J +4 more
europepmc +1 more source