Optimized green fluorescent protein fused to FoF1-ATP synthase for single-molecule FRET using a fast anti-Brownian electrokinetic trap [PDF]
, 2016 Observation times of freely diffusing single molecules in solution are limited by the photophysics of the attached fluorescence markers and by a small observation volume in the femtolitre range that is required for a sufficient signal-to-background ratio. To extend diffusion-limited observation times through a confocal detection volume, A. E. Cohen and
arxiv +1 more source
Journal of Biological Chemistry, 2002 Prostaglandin E2(PGE2) production involves the activity of a multistep biosynthetic pathway. The terminal components of this cascade, two PGE2 synthases (PGES), have very recently been identified as glutathione-dependent proteins.
R. Han, S. Tsui, Terry J. Smith
semanticscholar +1 more source
Sensing and Imaging Agents for Cyclooxygenase Enzyme
ChemMedChem, Volume 20, Issue 3, February 1, 2025.This concept explores the latest developments in COX‐2‐specific fluorescent probes for cancer imaging spotlighting their role in enhancing fluorescence upon binding to COX‐2. These probes provide accurate imaging of tumors and inflamed tissues which highlight the importance of COX‐2 as a critical cancer biomarker.
Subba Rao Cheekatla +3 more
wiley +1 more source
Asymmetry of rotational catalysis of single membrane-bound F0F1-ATP synthase [PDF]
arXiv, 2007 Erroneous submission in violation of copyright removed by arXiv admin.
arxiv
Journal of Biological Chemistry, 1999 Arg-120 is located near the mouth of the hydrophobic channel that forms the cyclooxygenase active site of prostaglandin endoperoxide H synthases (PGHSs)-1 and -2. Replacement of Arg-120 of ovine PGHS-1 with a glutamine increases the apparentK m of PGHS-1
C. Rieke +3 more
semanticscholar +1 more source
Synthetic Biology in Leishmaniasis: Design,simulation and validation of constructed Genetic circuit [PDF]
arXiv, 2013 Building circuits and studying their behavior in cells is a major goal of systems and synthetic biology. Synthetic biology enables the precise control of cellular states for systems studies, the discovery of novel parts, control strategies, and interactions for the design of robust synthetic systems. To the best of our knowledge,there are no literature
arxiv
Rapid Induction of Prostaglandin Endoperoxide Synthase in Rat Preovulatory Follicles by Luteinizing Hormone and cAMP is Blocked by Inhibitors of Transcription and Translation [PDF]
, 1989 Winona Y. L. Wong +3 more
openalex +1 more source
Regulatory conformational changes of the epsilon subunit in single FRET-labeled FoF1-ATP synthase [PDF]
arXiv, 2014 Subunit epsilon is an intrinsic regulator of the bacterial FoF1-ATP synthase, the ubiquitous membrane-embedded enzyme that utilizes a proton motive force in most organisms to synthesize adenosine triphosphate (ATP). The C-terminal domain of epsilon can extend into the central cavity formed by the alpha and beta subunits, as revealed by the recent X-ray
arxiv
Tyrosine 385 of prostaglandin endoperoxide synthase is required for cyclooxygenase catalysis
Journal of Biological Chemistry, 1990 There are spectral and biochemical data suggesting that a tyrosine group(s) is involved in the cyclooxygenase reaction catalyzed by prostaglandin endoperoxide (PGH) synthase. Treatment with tetranitromethane, a reagent which nitrates tyrosine residues, abolishes cyclooxygenase activity, but this inactivation can be largely prevented by competitive ...
David L. DeWitt +3 more
openaire +3 more sources
Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy [PDF]
arXiv, 2017 Cytochrome C oxidase and FoF1-ATP synthase constitute complex IV and V, respectively, of the five membrane-bound enzymes in mitochondria comprising the respiratory chain. These enzymes are located in the inner mitochondrial membrane (IMM), which exhibits large invaginations called cristae.
arxiv