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Beyond olfaction: New insights into human odorant binding proteins. [PDF]
Chen M +5 more
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MALDI spatial proteomics: a mini review of approaches and techniques.
Lopez A, Hummon AB.
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Biochemical Society Transactions, 2010
In the present article, we describe the two standard high-throughput methods for identification of protein complexes: two-hybrid screens and TAP (tandem affinity purification) tagging. These methods have been used to characterize the interactome of Saccharomyces cerevisiae, showing that the majority of proteins are part of complexes, and that complexes
Williamson, Mike P. +1 more
openaire +3 more sources
In the present article, we describe the two standard high-throughput methods for identification of protein complexes: two-hybrid screens and TAP (tandem affinity purification) tagging. These methods have been used to characterize the interactome of Saccharomyces cerevisiae, showing that the majority of proteins are part of complexes, and that complexes
Williamson, Mike P. +1 more
openaire +3 more sources
DISCOVERING PROTEIN–PROTEIN INTERACTIONS
Journal of Bioinformatics and Computational Biology, 2004The ongoing genomics and proteomics efforts have helped identify many new genes and proteins in living organisms. However, simply knowing the existence of genes and proteins does not tell us much about the biological processes in which they participate. Many major biological processes are controlled by protein interaction networks.
See-Kiong, Ng, Soon-Heng, Tan
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Analyzing Protein–Protein Interaction Networks
Journal of Proteome Research, 2012The advent of the "omics" era in biology research has brought new challenges and requires the development of novel strategies to answer previously intractable questions. Molecular interaction networks provide a framework to visualize cellular processes, but their complexity often makes their interpretation an overwhelming task.
Koh GC +4 more
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