Results 311 to 320 of about 583,434 (343)
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Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports
Cold Spring Harbor Protocols, 2006INTRODUCTIONThe following protocol describes the synthesis of short linear peptides, or peptide pools, on modified cellulose membranes, and the detection of their protein-binding partners. Peptides are synthesized from their carboxyl termini using Fmoc-amino acid derivatives.
Ronald, Frank, Stefan, Dübel
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Cold Spring Harbor Protocols, 2006
INTRODUCTIONThe most difficult aspect of using an array approach for a high-throughput study is maintaining the array’s viability while avoiding contamination of the plates. This protocol describes three different storage methods for the array. Frozen stocks are the most permanent method and are an absolute requirement for maintaining a record of the ...
Tony R, Hazbun, John P, Miller
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INTRODUCTIONThe most difficult aspect of using an array approach for a high-throughput study is maintaining the array’s viability while avoiding contamination of the plates. This protocol describes three different storage methods for the array. Frozen stocks are the most permanent method and are an absolute requirement for maintaining a record of the ...
Tony R, Hazbun, John P, Miller
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Protein analysis by isoelectric focusing in a capillary array with an absorption imaging detector
Journal of Chromatography B: Biomedical Sciences and Applications, 1995Isoelectric focusing (IEF) was successfully performed in capillary arrays with up to four capillaries. Separated proteins in the capillary array were detected by an UV absorption imaging detector. The whole analysis time for all samples in the capillary array was only 3 min due to the real-time imaging detector.
J, Wu, J, Pawliszyn
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Protein chip array profiling analysis of sera from neuroblastoma patients
Cancer Letters, 2005Neuroblastoma, the most common extracranial solid tumour in children, is characterised by highly heterogeneous clinical behaviour; patients are stratified into risk categories according to a combination of clinical and biological markers. However, identifying non-invasive prognostic markers predicting outcome independently from current risk ...
Valérie, Combaret +6 more
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Single layer linear array of microbeads for multiplexed analysis of DNA and proteins
Biosensors and Bioelectronics, 2014In this study, a microfluidic platform was developed to generate single layer, linear array of microbeads for multiplexed high-throughput analysis of biomolecules. The microfluidic device is comprised of eight microbead-trapping units, where microbeads were immobilized in a linear array format by the exertion of a negative pressure in the control ...
Wanqing, Yue +9 more
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Analysis of protein tyrosine kinase expression in melanocytic lesions by tissue array
Journal of Cutaneous Pathology, 2003Background: It has been proposed that melanoma progression involves a multistep process from benign nevi (BN), dysplastic nevi (DN), radial and vertical growth phase melanoma (MM) to metastatic melanoma (MMM). Protein tyrosine kinases (PTKs) may participate in this progression.Methods: Tissue microarray blocks of 89 melanocytic lesions were evaluated
Steven S, Shen +3 more
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Experimental Design & Analysis of Protein Array Data: Applying Methods from cDNA Arrays.
Blood, 2004Abstract It appears that a number of recent manuscripts using protein microarray technology are using equivalent analysis procedures that the gene-expression microarray community implemented in their infancy. That is, utilizing a classic reference design such that the ratio of the sample of interest to a reference sample is the response ...
Irene Ghobrial +2 more
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[Method of antibody analysis based on visual protein array].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2010To establish a novel method for antibody analysis based on visual protein microarray.The antibodies spotted on the aldehyde-modified slides were incubated with corresponding antigens labeled by biotin, followed by silver enhancement detection method, and the chromogenic images were scanned by CCD camera.The affinity and specificity of each antibody was
Zhe, Yu +7 more
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Microwestern Arrays for Systems-Level Analysis of SH2 Domain-Containing Proteins
2017The Microwestern Array (MWA) method combines the scalability and miniaturization afforded by the Reverse Phase Lysate Array (RPLA) approach with the electrophoretic separation characteristic of the Western blot. This technology emulates the creation of an array of small Western blots on a single sheet of nitrocellulose allowing for the sensitive and ...
Mark F, Ciaccio, Richard B, Jones
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