Results 31 to 40 of about 4,061,795 (267)

Baculovirus Expression Vectors that Incorporate the Foreign Protein into Viral Occlusion Bodies

open access: yesBioTechniques, 2003
Current baculovirus expression systems typically produce soluble proteins that accumulate within the infected insect cell or are secreted into the growth medium.
Y.H. Je   +8 more
doaj   +1 more source

Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes

open access: yesiScience, 2022
Summary: RNA localization and biomolecular condensate formation are key biological strategies for organizing the cytoplasm and generating cellular polarity. In Xenopus oocytes, RNAs required for germ layer patterning localize in biomolecular condensates,
Sarah E. Cabral   +2 more
doaj   +1 more source

Regulation of processing body formation in the budding yeast "Saccharomyces cerevisiae" [PDF]

open access: yes, 2014
The life cycle of mRNA, from transcription to decay, is a tightly regulated biological process. mRNA is an essential molecule that the cell utilizes to alter gene expression. Degradation is one process through which the cell can regulate mRNA levels.
Weidner, Julie
core   +1 more source

Hsp90 Breaks the Deadlock of the Hsp70 Chaperone System [PDF]

open access: yes, 2018
Protein folding in the cell requires ATP-driven chaperone machines such as the conserved Hsp70 and Hsp90. It is enigmatic how these machines fold proteins.
Mayer, Matthias   +5 more
core   +2 more sources

Protein folding and the formation of inclusion bodies

open access: yes浙江大学学报. 农业与生命科学版, 2004
Newly synthesized polypeptide chains must be folded and assembled into unique three-dimensional structures in order to become functionally active. In the folding process, many misfolded proteins maybe produced and thus accumulate as aggregates.
JING Ming-yan, SUN Jian-yi
doaj   +1 more source

Is there a body protein reserve? [PDF]

open access: yesProceedings of the Nutrition Society, 1982
Evidence from experimentally protein-depleted dogs and adult human volunteers shows that about 20-25% of total body protein can be lost without fatality. Muscle protein is used to protect visceral proteins. In obese adults 15% of body protein (together with a large amount of fat) can be lost without ill effects.
openaire   +2 more sources

Expressed protein ligation for a large dimeric protein [PDF]

open access: yes, 2011
Expressed protein ligation (EPL) is a protein engineering tool for post-translational ligation of protein or peptide fragments. This technique allows modification of specific parts of proteins, opening possibilities for incorporating probes for ...
NMR Spectroscopy   +11 more
core   +1 more source

Large scale deletion and rebalancing within the k1C kafirin family in sorghum

open access: yesFrontiers in Plant Science
Sorghum bicolor (L.) Moench (sorghum) is cultivated as food for humans and livestock and is valued for its low input requirements. However, sorghum grain protein is deficient in the essential amino acid lysine and has poor protein digestibility.
Tyler W. Ferris   +7 more
doaj   +1 more source

Learning about protein solubility from bacterial inclusion bodies

open access: yesMicrobial Cell Factories, 2009
The progressive solving of the conformation of aggregated proteins and the conceptual understanding of the biology of inclusion bodies in recombinant bacteria is providing exciting insights on protein folding and quality.
García-Fruitós Elena   +3 more
doaj   +1 more source

Pull-Down Into Active Inclusion Bodies and Their Application in the Detection of (Poly)-Phosphates and Metal-Ions

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Inclusion bodies are typically ignored as they are considered unwanted protein waste generated by prokaryotic host cells during recombinant protein production or harmful protein inclusions in human cell biology.
Eva Hrabarova   +7 more
doaj   +1 more source

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