Results 131 to 140 of about 6,634,608 (387)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Highly conserved ion binding sites are not all functionally relevant in mouse KCC4

Frontiers in Molecular Biosciences
IntroductionThe potassium chloride cotransporter 4 (KCC4) is expressed in various tissues and plays an important role in distal renal acidification and hearing development.
Lisa Becker, Jens Hausmann, Rieke Wellpott, Anna-Maria Hartmann, Anna-Maria Hartmann   +4 more
doaj   +1 more source

A Conformational Change in C-Reactive Protein Enhances Leukocyte Recruitment and Reactive Oxygen Species Generation in Ischemia/Reperfusion Injury [PDF]

, 2018
Jan Thiele, Johannes Zeller, Jurij Kiefer, David Braig, Sheena Kreuzaler, Yvonne Lenz, Lawrence A. Potempa, Florian Grahammer, Tobias B. Huber, Markus Huber‐Lang, Holger Bannasch, G. Björn Stark, Karlheinz Peter, Steffen U. Eisenhardt   +13 more
openalex   +1 more source

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source

Investigation of conformational changes of cathepsin B as a feasibility assessment for NaCl reduction in Jinhua ham

Food Science and Human Wellness
To regulate the sodium chloride content in Jinhua ham, the impact of NaCl on the activity and conformation of cathepsin B was investigated using spectroscopy and computational methods. The results showed that the activity of cathepsin B decreased with an
Shiqi Hu, Guanghong Zhou, Xinglian Xu, Wangang Zhang, Chunbao Li   +4 more
doaj   +1 more source

Protein folding tames chaos [PDF]

, 2013
Protein folding produces characteristic and functional three-dimensional structures from unfolded polypeptides or disordered coils. The emergence of extraordinary complexity in the protein folding process poses astonishing challenges to theoretical ...
Wei, Guo-Wei, Xia, Kelin
core  

Protein conformation and disease.

Veterinary research, 1996
In a number of neurodegenerative diseases, a change in the conformation of specific proteins occurs which is considered to be related to the development of the disease process. In general, a change in the protein conformation from a predominantly alpha-helical to a predominantly beta-sheet structure, due to genetic or environmental factors, leads to ...
openaire   +2 more sources

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

The Effects of Tea Polyphenols on the Emulsifying and Gelling Properties of Minced Lamb After Repeated Freeze–Thaw Cycles

Foods
Minced lamb remains one of the most produced meat products in the meat industry, across both the food service and retail sectors. Tea polyphenols (TPs), renowned for their diverse biological activities, are increasingly being employed as natural food ...
Xueyan Yun, Ganqi Yang, Limin Li, Ying Wu, Xujin Yang, Aiwu Gao   +5 more
doaj   +1 more source