Heterogeneous Nucleation in Protein Crystallization [PDF]
Biomimetics, 2023 Protein crystallization was first discovered in the nineteenth century and has been studied for nearly 200 years. Protein crystallization technology has recently been widely used in many fields, such as drug purification and protein structure analysis ...
Hao Liu, Yue Zhao, Jing Sun
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Protein Crystallization in a Microfluidic Contactor with Nafion®117 Membranes [PDF]
Membranes, 2021 Protein crystallization still remains mostly an empirical science, as the production of crystals with the required quality for X-ray analysis is dependent on the intensive screening of the best protein crystallization and crystal’s derivatization ...
M. Polino +9 more
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Yield of Protein Crystallization from Metastable Liquid–Liquid Phase Separation [PDF]
MoleculesPreparative protein crystallization is regarded as an economically sustainable protein purification alternative to chromatography in biotechnological downstream processing. However, protein crystallization is a not-well-understood process that is usually
Shamberia Thomas +2 more
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Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening [PDF]
Nanomaterials, 2023 Modifying crystallization plates can significantly impact the success rate and quality of protein crystal growth, making it a helpful strategy in protein crystallography.
Qin Yang +5 more
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Benchmarking protein language models for protein crystallization [PDF]
Scientific ReportsThe problem of protein structure determination is usually solved by X-ray crystallography. Several in silico deep learning methods have been developed to overcome the high attrition rate, cost of experiments and extensive trial-and-error settings, for ...
Raghvendra Mall +4 more
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Cell-free protein crystallization for nanocrystal structure determination [PDF]
Scientific Reports, 2022 In-cell protein crystallization (ICPC) has been investigated as a technique to support the advancement of structural biology because it does not require protein purification and a complicated crystallization process.
Satoshi Abe +7 more
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Intracellular protein crystallization in living insect cells [PDF]
FEBS Open BioCrystallization of recombinant proteins in living cells is an emerging approach complementing conventional crystallization techniques. Homogeneous microcrystals well suited for serial diffraction experiments at X‐ray free‐electron lasers and synchrotron ...
Robert Schönherr +8 more
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Automation of protein crystallization scaleup via Opentrons-2 liquid handling [PDF]
SLAS TechnologyIn this study we present an approach for optimizing protein crystallization trials at the multi-microliter scale utilizing the Opentrons-2 liquid handling robot.
Jacob B. DeRoo +6 more
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Exploring the potential of a bioassembler for protein crystallization in space [PDF]
npj MicrogravityProtein crystallization holds paramount significance in structural biology, serving as a pivotal technique for unveiling the three-dimensional (3D) architecture of proteins.
Christopher MacCarthy +18 more
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Rational Introduction of Electrostatic Interactions at Crystal Contacts to Enhance Protein Crystallization of an Ene Reductase [PDF]
BiomoleculesProtein crystallization is an alternative to well-established but cost-intensive and time-consuming chromatography in biotechnological processes, with protein crystallization defined as an essential unit operation for isolating proteins, e.g., active ...
Brigitte Walla +5 more
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