Results 11 to 20 of about 178,624 (341)

Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking

FEBS Open Bio
Protein crystallogenesis represents a key step in X‐ray crystallography studies that employ co‐crystallization and ligand soaking for investigating ligand binding to proteins.
Barbora Kaščáková, Anna Koutská, Michaela Burdová, Petra Havlíčková, Ivana Kutá Smatanová   +4 more
doaj   +3 more sources

Fusion-protein-assisted protein crystallization [PDF]

Acta Crystallographica Section F Structural Biology Communications, 2015
Fusion proteins can be used directly in protein crystallization to assist crystallization in at least two different ways. In one approach, the ‘heterologous fusion-protein approach’, the fusion partner can provide additional surface area to promote ...
Kobe, Bostjan, Ve, Thomas, Williams, Simon J.   +2 more
core   +5 more sources

Integrated <i>In Vitro</i>/<i>In Silico</i> Uncertainty Quantification Method for Protein Crystallization Models. [PDF]

Ind Eng Chem Res
Pessina D, Calderon De Anda J, Heffernan C, Heng JYY, Papathanasiou MM.   +4 more
europepmc   +2 more sources

Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications

Molecules, 2021
In this work, we combined electrically-conductive graphene oxide and a sodium alginate-caffeic acid conjugate, acting as a functional element, in an acrylate hydrogel network to obtain multifunctional materials designed to perform multiple tasks in ...
Giuseppe Cirillo, Elvira Pantuso, Manuela Curcio, Orazio Vittorio, Antonella Leggio, Francesca Iemma, Giovanni De Filpo, Fiore Pasquale Nicoletta   +7 more
doaj   +1 more source

Microgravity protein crystallization. [PDF]

NPJ Microgravity, 2015
AbstractOver the past 20 years a variety of technological advances in X-ray crystallography have shortened the time required to determine the structures of large macromolecules (i.e., proteins and nucleic acids) from several years to several weeks or days.
McPherson A, DeLucas LJ.
europepmc   +6 more sources

Theoretical and experimental investigation of protein crystal nucleation in pores and crevices

IUCrJ, 2021
The nucleation ability of pores is explained using the equilibration between the cohesive energy maintaining the integrity of a crystalline cluster and the destructive energy tending to tear it up.
Christo Nanev, Lata Govada, Naomi E. Chayen   +2 more
doaj   +1 more source

Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals

Crystals, 2020
Flavin-dependent halogenases chlorinate or brominate their substrates in an environmentally friendly manner, only requiring the cofactor reduced flavin adenine dinucleotide (FADH2), oxygen, and halide salts.
Ann-Christin Moritzer, Tina Prior, Hartmut H. Niemann   +2 more
doaj   +1 more source

Through Diffusion Measurements of Molecules to a Numerical Model for Protein Crystallization in Viscous Polyethylene Glycol Solution

Crystals, 2022
Protein crystallography has become a popular method for biochemists, but obtaining high-quality protein crystals for precise structural analysis and larger ones for neutron analysis requires further technical progress.
Hiroaki Tanaka, Rei Utata, Keiko Tsuganezawa, Sachiko Takahashi, Akiko Tanaka   +4 more
doaj   +1 more source

Protein—protein crystal‐packing contacts [PDF]

Protein Science, 1997
AbstractProtein‐protein contacts in monomeric protein crystal structures have been analyzed and compared to the physiological protein‐protein contacts in oligomerization. A number of features differentiate the crystal‐packing contacts from the natural contacts occurring in multimeric proteins. The area of the protein surface patches involved in packing
CARUGO, OLIVIERO ITALO, Argos P.
openaire   +2 more sources

A Searchable Database of Crystallization Cocktails in the PDB: Analyzing the Chemical Condition Space

Patterns, 2020
Summary: Nearly 90% of structural models in the Protein Data Bank (PDB), the central resource worldwide for three-dimensional structural information, are currently derived from macromolecular crystallography (MX).
Miranda L. Lynch, Max F. Dudek, Sarah E.J. Bowman   +2 more
doaj   +1 more source