Results 231 to 240 of about 178,624 (341)

Crystallization and preliminary crystallographic analysis of an Ig-domain-encompassing fragment of the giant adhesion protein SiiE fromSalmonella enterica [PDF]

, 2011
Karina Sturm, Martin H. Griessl, Carolin Wagner, Jörg Deiwick, Michael Hensel, Yves A. Muller   +5 more
openalex   +1 more source

Structure‐Guided Engineering of a Promiscuous O‐Methyltransferase for a SAM Regeneration Biocatalysis Platform of Methylated Pharmaceuticals

Advanced Science, EarlyView.
A substrate promiscuous and regioselective O‐methyltransferase, SmOMT, is functionally and structurally characterized. A double mutant, SmOMTE152A/I306A, exhibited enhanced catalytic activity. By coupling this mutant with a mutant halide methyltransferase, AtHMTV140T, for SAM regeneration, a superior artificial fusion enzyme, AtHMTV140T‐L95‐SmOMTE152A ...
Xiran Xiong, Jun Song, Shihan Li, Lu Jin, Qi He, Baohui Zhang, Yan Cao, Shanyong Yi, Yanfang Yang, Xiang Li, Juan Li, Wei Huang   +11 more
wiley   +1 more source

CHiMP: deep-learning tools trained on protein crystallization micrographs to enable automation of experiments. [PDF]

Acta Crystallogr D Struct Biol
King ONF, Levik KE, Sandy J, Basham M.
europepmc   +1 more source

Enhanced Recognition via Defect Engineered Amorphous Metal–Organic Framework for Sensitive and Stable Pesticide Biosensor

Advanced Science, EarlyView.
A defect‐engineered assembly strategy is reported for the in situ encapsulation of acetylcholinesterase (AChE) into amorphous MOF‐74, endowing the composite with superior catalytic activity and target recognition capability. Leveraging these structural advantages, a sensitive and stable biosensor is constructed for the detection of paraoxon pesticide ...
Changshun Su, Xiangyu Zhai, Meng Zhang, Mengxue Li, Xinru Zhang, Chunyan Sun, Xu Yan, Hongxia Li   +7 more
wiley   +1 more source

Systematic enhancement of protein crystallization efficiency by bulk lysine-to-arginine (KR) substitution. [PDF]

Protein Sci
Banayan NE, Loughlin BJ, Singh S, Forouhar F, Lu G, Wong KH, Neky M, Hunt HS, Bateman LB, Tamez A, Handelman SK, Price WN, Hunt JF.   +12 more
europepmc   +1 more source

A defined protein–detergent–lipid complex for crystallization of integral membrane proteins: The cytochrome b ₆ f complex of oxygenic photosynthesis

, 2003
Huamin Zhang, Genji Kurisu, Janet L. Smith, William A. Cramer   +3 more
openalex   +1 more source

Protein crystallization promotes type 2 immunity and is reversible by antibody treatment [PDF]

, 2019
Emma K. Persson, Kenneth Verstraete, Ines Heyndrickx, Elien Gevaert, Helena Aegerter, Jean-Michel Percier, Kim Deswarte, Koen H. G. Verschueren, Ann Dansercoer, Delphine Gras, Pascal Chanez, Claus Bachert, Amanda Gonçalves, Hanne Van Gorp, Hans de Haard, Christophe Blanchetot, Michael Saunders, Hamida Hammad, Savvas N. Savvides, Bart N. Lambrecht   +19 more
openalex   +1 more source

Visualizing and Quantifying Impact with Mechanochromic Sensing Paints Based on Self‐Assembled Polydiacetylene‐Silk Core‐Shell Vesicles

Advanced Science, EarlyView.
Tracking physical impacts is important in many fields. Self‐assembled microparticles made from polydiacetylene and silk fibroin that change color from blue to red when hit can provide an alternative approach to traditional mechanical transducers, quantitatively visualizing impact with responses ranging from <100 to 770 N.
Marco Lo Presti, Giulia Guidetti, Elisabetta Ruggeri, Terri Lyne Carrington, Fiorenzo G. Omenetto   +4 more
wiley   +1 more source

High-throughput structure determination of an intrinsically disordered protein using cell-free protein crystallization. [PDF]

Proc Natl Acad Sci U S A
Kojima M, Abe S, Furuta T, Hirata K, Yao X, Kobayashi A, Kobayashi R, Ueno T.   +7 more
europepmc   +1 more source

Micro‐Organ Chip Deciphers Tumor‐Derived G‐CSF as Remote Commander of Lung Pre‐Metastatic Niche via VEGFA‐KDR Cascade

Advanced Science, EarlyView.
Breast cancer reprograms the lung into a receptive pre‐metastatic niche via a novel G‐CSF–VEGFA–KDR signaling axis. Using a micro‐organ chip that enables contact‐independent coculture, this study uncovers how tumor‐secreted G‐CSF activates lung capillary KDR to drive angiogenesis and prime the soil for metastasis—without direct tumor contact.
Jingxin Zhang, Xiaoying Huang, Lingchuan Ma, Zixing Chen, Tianyao Li, Lijian Wang, Yutong Guo, Hu Xu, Junqi Li, Jiang‐Jiang Qin, Xiang Wang, Yuhong Cao, Kai Miao   +12 more
wiley   +1 more source