Results 291 to 300 of about 178,624 (341)

Efficient protein crystallization

Journal of Structural Biology, 2003
High-throughput molecular biology and crystallography advances have placed an increasing demand on crystallization, the one remaining bottleneck in macromolecular crystallography. This paper describes three experimental approaches, an incomplete factorial crystallization screen, a high-throughput nanoliter crystallization system, and the use of a ...
Lawrence J, DeLucas, Terry L, Bray, Lisa, Nagy, Debbie, McCombs, Nikolai, Chernov, David, Hamrick, Larry, Cosenza, Alexander, Belgovskiy, Brad, Stoops, Arnon, Chait   +9 more
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Protein crystal microspectrophotometry

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2011
Single crystal microspectrophotometry has emerged as a valuable technique for monitoring molecular events that take place within protein crystals, thus tightly coupling structure to function. Absorption and fluorescence spectra, ligand binding affinities and kinetic constants can be determined, allowing i) the definition of the experimental conditions ...
RONDA, Luca, BRUNO, Stefano, BETTATI, Stefano, MOZZARELLI, Andrea   +3 more
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Protein crystallization: from purified protein to diffraction-quality crystal

Nature Methods, 2008
Determining the structure of biological macromolecules by X-ray crystallography involves a series of steps: selection of the target molecule; cloning, expression, purification and crystallization; collection of diffraction data and determination of atomic positions.
Naomi E, Chayen, Emmanuel, Saridakis
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Membrane protein crystallization

Journal of Structural Biology, 2003
The need for high-resolution structure information on membrane proteins is immediate and growing. Currently, the only reliable way to get it is crystallographically. The rate-limiting step from protein to structure is crystal production. An overview of the current ideas and experimental approaches prevailing in the area of membrane protein ...
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Nucleation in Protein Crystallization

Acta Crystallographica Section D Biological Crystallography, 1998
In this paper protein crystallization is regarded as a process starting with phase separation in a two-component system. The nucleation time of a lysozyme solution is measured by recording the NMR spectra of crystallizing solutions as a function of time.
J, Drenth, C, Haas
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High-throughput protein crystallization

Journal of Structural Biology, 2003
High-throughput structural biology is a focus of a number of academic and pharmaceutical laboratories around the world. The use of X-ray crystallography in these efforts is critically dependent on high-throughput protein crystallization. The application of current protocols yields crystal leads for approximately 30% of the input proteins and well ...
Raymond, Hui, Aled, Edwards
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Crystallization of Membrane Proteins

2013
The crystallization of membrane proteins is an essential technique for the determination of atomic models of three-dimensional structures by X-ray crystallography. The compositions of solutions of purified membrane proteins are altered, so as to transiently induce supersaturation, a requirement for crystal nucleation and growth.
Florian G, Müller, C Roy D, Lancaster
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Shaped protein single crystals

Acta Crystallographica Section D Biological Crystallography, 1995
The formation of protein single crystals grown with the shape controlled by the geometry of the capillary used as a growth cell is presented. The shaped crystals show strong birefringence under crossed nicols and diffract as single crystals up to 1.74 A.
J M, García-Ruiz, A, Moreno, A, Parraga, M, Coll   +3 more
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Seeding Protein Crystallization with Cross-Linked Protein Crystals

Crystal Growth & Design, 2018
Protein crystallization is of great importance because protein crystals have a number of different important applications, including large-scale purification of proteins, determination of protein structure, nanoparticle preparation, and theoretical studies of crystallization.
Er-Kai Yan, Feng-Zhu Zhao, Chen-Yan Zhang, Xue-Zhou Yang, Miao Shi, Jin He, Ya-Li Liu, Yue Liu, Hai Hou, Da-Chuan Yin   +9 more
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Effective Protein Crystallization Using Crystal Hysteresis

Japanese Journal of Applied Physics, 2003
We have developed a new method for protein crystallization, crystal hysteresis, that utilizes slow-cooling and recooling techniques. Slow cooling of a saturated solution enables precise control of supersaturation in the solution and also allows reuse of the solution because it maintains its composition.
Hiroaki Adachi, Kazufumi Takano, Masashi Yoshimura, Yusuke Mori, Takatomo Sasaki   +4 more
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