Results 31 to 40 of about 178,624 (341)

Acoustic combinatorial crystallization generates huge 9216 crystallization library from 64 building blocks [PDF]

Structural Dynamics
We present a novel method for protein crystallization: acoustic combinatorial crystallization. In this approach, crystallization cocktails are assembled directly on crystallization labware using acoustic liquid handling, without relying on a pre-defined ...
Alexei Soares
doaj   +1 more source

Functionalization of the BCL6 BTB domain into a noncovalent crystallization chaperone

IUCrJ, 2021
The production of diffraction-quality protein crystals is challenging and often requires bespoke, time-consuming and expensive strategies. A system has been developed in which the BCL6 BTB domain acts as a crystallization chaperone and promiscuous ...
Thomas Zacharchenko, Stephanie Wright
doaj   +1 more source

Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants [PDF]

, 2005
The crystallization of three catalytically inactive mutants of penicillin Vacylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported.
Brannigan, J A, Chandra, P M, Dodson, G G, Prabhune, A, Pundle, A, Suresh, C G, Turkenburg, J P   +6 more
core   +2 more sources

Preparation and target protease identification of a cyanobacterial serine protease inhibitor, arthropin

Shanghai Jiaotong Daxue xuebao. Yixue ban, 2023
Objective·To prepare a high-purity cyanobacterial serine protease inhibitor, screen its target proteases, and detect its inhibitory activity.Methods·A novel serine protease inhibitor from Arthrospira platensis was identified in the Expanded Human Oral ...
XU Jiawei, ZHOU Aiwu, YANG Yufeng
doaj   +1 more source

Optimization of crystal nucleation close to a metastable fluid-fluid phase transition [PDF]

, 2015
The presence of a metastable fluid-fluid critical point is thought to dramatically influence the crystallization pathway, increasing the nucleation rate by many orders of magnitude over the predictions of classical nucleation theory.
Buldyrev, Sergey V., Franzese, Giancarlo, Reguera, David, Stanley, Harry Eugene, Wedekind, Jan, Xu, Limei   +5 more
core   +1 more source

A Double Emulsion-Based, Plastic-Glass Hybrid Microfluidic Platform for Protein Crystallization

Micromachines, 2015
This paper reports the design and construction of a plastic-glass hybrid microfluidic platform for performing protein crystallization trials in nanoliter double emulsions.
Deyong Zhu, Xiaohu Zhou, Bo Zheng
doaj   +1 more source

On the Quality of Protein Crystals Grown under Diffusion Mass-transport Controlled Regime (I)

Crystals, 2020
It has been previously shown that the diffraction quality of protein crystals strongly depends on mass transport during their growth. In fact, several studies support the idea that the higher the contribution of the diffusion during mass transport, the ...
José A. Gavira, Fermín Otálora, Luis A. González-Ramírez, Emilio Melero, Alexander E.S. van Driessche, Juan Manuel García-Ruíz   +5 more
doaj   +1 more source

Lysozyme crystallization in hydrogel media under ultrasound irradiation

Ultrasonics Sonochemistry, 2022
Sonocrystallization implies the application of ultrasound radiation to control the nucleation and crystal growth depending on the actuation time and intensity.
Mariia Savchenko, Manuel Hurtado, Modesto T. Lopez-Lopez, Guillermo Rus, Luis Álvarez de Cienfuegos, Juan Melchor, José A. Gavira   +6 more
doaj   +1 more source

Characterization of Aptamer-Protein Complexes by X-ray Crystallography and Alternative Approaches [PDF]

, 2012
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms.
Baugh, Bauke W. Dijkstra, Bing, Bock, Cao, Chayen, Convery, Doudna, Ellington, Friedmann, Garber, Hauke Smidt, Hermann, Hianik, Hoggan, Hollis, Horn, Huang, Huang, Hwang, Jiang, Johan Hekelaar, John van der Oost, Kaur, Ke, Kelly, Kikin, Krauss, Kwan, Laing, Lebruska, Lee, Long, Lupold, Macaya, Mark Levisson, Mascini, McPherson, Mehta, Miyakawa, Moorthy, Murai, Nix, Nomura, Orlova, Padmanabhan, Padmanabhan, Paige, Parisien, Poniková, Reinemann, Reinstein, Renault, Rivas, Rowsell, Ruigrok, Sekiya, Shum, Skrzypczak-Jankun, Snyder, Someya, Stoltenburg, Sugiyama, Sussman, Tereshko, Tuerk, Vincent J. B. Ruigrok, Wang, Wilson, Win, Wochner, Yan, Yee, Zuker   +73 more
core   +6 more sources

Refolding, Characterization, and Preliminary X-ray Crystallographic Studies on the Campylobacter concisus Plasmid-Encoded Secreted Protein Csep1p Associated with Crohn’s Disease

Crystals, 2018
Colonization of Campylobacter concisus in the gastrointestinal tract can lead to the development of inflammatory bowel disease (IBD). Plasmid-encoded C.
Mohammad Mizanur Rahman, Bradley Goff, Li Zhang, Anna Roujeinikova   +3 more
doaj   +1 more source