Results 41 to 50 of about 178,624 (341)

Structural transitions of monoolein bicontinuous cubic phase induced by inclusion of protein lysozyme solutions [PDF]

, 2006
Inclusion of protein lysozyme molecules in lipidic monoolein cubic phase induces a transition from a $\rm Pn\bar{3}m$ structure to $\rm Im\bar{3}m$ one.
M. Ataka, S. Maki, S. T. Hyde, S. Tanaka
core   +2 more sources

Crystallization and preliminary crystallographic analysis of the DNA gyrase B protein from B-stearothermophilus [PDF]

, 1996
DNA gyrase B (GyrB) from B. stearothermophilus has been crystallized in the presence of the non-hydrolyzable ATP analogue, 5'-adenylpl-beta-gamma-imidodiphosphate (ADPNP), by the dialysis method.
Brannigan, J A, Subramanya, H S, Tsai, F T F, Wigley, D B, Wilkinson, A J   +4 more
core   +2 more sources

In Situ Microscopy with Real-Time Image Analysis Enables Online Monitoring of Technical Protein Crystallization Kinetics in Stirred Crystallizers

Crystals
Controlling protein crystallization processes is essential for improving downstream processing in biotechnology. This study investigates the combination of machine learning-based image analysis and in situ microscopy for real-time monitoring of protein ...
Julian Mentges, Daniel Bischoff, Brigitte Walla, Dirk Weuster-Botz   +3 more
doaj   +1 more source

The crystallization of asymmetric patchy models for globular proteins in solution

, 2013
Asymmetric patchy particle models have recently been shown to describe the crystallization of small globular proteins with near quantitative accuracy. Here, we investigate how asymmetry in patch geometry and bond energy generally impact the phase diagram
Charbonneau, Patrick, Fusco, Diana
core   +2 more sources

Of bits and bugs [PDF]

, 2010
Pur-α is a nucleic acid-binding protein involved in cell cycle control, transcription, and neuronal function. Initially no prediction of the three-dimensional structure of Pur-α was possible.
Graebsch, Almut, Kostrewa, Dirk, Niessing, Dierk, Roche, Stéphane, Söding, Johannes   +4 more
core   +1 more source

Plastic-embedded protein crystals [PDF]

Journal of Synchrotron Radiation, 2006
Rapid vitrification followed by the replacement of the vitrified water by a solvent (freeze substitution) and then resin is a widely used procedure for preparing biological samples for electron microscopy. The resulting plastic-embedded samples permit convenient room-temperature sectioning (microtomy) and can yield well preserved cellular structures ...
Ravelli, R., Haselmann-Weiss, U., McGeehan, John, McCarthy, A., Marquez, J., Antony, C., Frangakis, A., Stranzl, G.   +7 more
openaire   +3 more sources

Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

Crystals, 2017
Three-dimensional protein molecule structures are essential for acquiring a deeper insight of the human genome, and for developing novel protein-based pharmaceuticals. X-ray diffraction studies of such structures require well-diffracting protein crystals.
Christo N. Nanev
doaj   +1 more source

Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A [PDF]

, 1999
The key response-regulator gene of sporulation, spo0A, has been cloned from Bacillus stearothermophilus and the encoded protein purified. The DNA-binding and phospho-acceptor domains of Spo0A have been prepared by tryptic digestion of the intact protein ...
Barak, I, Brannigan, J A, Brown, D P, Lewis, R J, Muchova, K, Offen, W A, Wilkinson, A J, Youngman, P   +7 more
core   +1 more source

Precipitant-Free Crystallization of Protein Molecules Induced by Incision on Substrate

Crystals, 2017
Nucleation of protein crystals has been shown to be facilitated by substrates decorated with both nano- to micro-scale hierarchical undulations and spatially varying surface potential. In fact, on such surfaces, several proteins were found to crystallize
Anindita Sengupta Ghatak, Gaurav Rawal, Animangsu Ghatak   +2 more
doaj   +1 more source

Crystallization and preliminary X-ray analysis of neoagarobiose hydrolase from Saccharophagus degradans 2-40 [PDF]

, 2009
Many agarolytic bacteria degrade agar polysaccharide into the disaccharide unit neoagarobiose [O-3,6-anhydro-α-L-galactopyranosyl-(1→3)-D-galactose] using various β-agarases.
Araki, Collaborative Computational Project, Number 4, Day, Dong Hae Shin, Ekborg, In-Geol Choi, Jancarik, Jina Jung, Jonas Yun Lee, Kyoung Heon Kim, Lee, Matthews, Otwinowski, Park, Renn, Saeyoung Lee, Sugano, Sung Chul Ha, Suzuki   +18 more
core   +2 more sources