Results 51 to 60 of about 178,624 (341)
Anaerobic crystallization of proteins [PDF]
Biophysical Reviews, 2017 Crystallization has been a bottleneck in the X-ray crystallography of proteins. Although many techniques have been developed to overcome this obstacle, the impurities caused by chemical reactions during crystallization have not been sufficiently considered.
Miki Senda, Toshiya Senda
openaire +2 more sources
IUCrJ, 2014 An emulsion-based serial crystallographic technology has been developed, in which nanolitre-sized droplets of protein solution are encapsulated in oil and stabilized by surfactant. Once the first crystal in a drop is nucleated, the small volume generates
Michael Heymann +6 more
doaj +1 more source
IUCrJ, 2021 Lipidic cubic phase (LCP) crystallization methods have been essential in obtaining crystals of certain membrane proteins, particularly G-protein-coupled receptors.
Thomas Cleveland IV +5 more
doaj +1 more source
Crystallization and preliminary X-ray analysis of the sporulation factor SpoIIAA in its native and phosphorylated forms [PDF]
, 2001 Sporulation in Bacillus begins with an asymmetric cell division producing two progeny with identical chromosomes but different developmental fates. As such, it is a simple example of cellular differentiation.
Brannigan, J A +3 more
core +1 more source
Enhancement of Lysozyme Crystallization Using DNA as a Polymeric Additive
Crystals, 2019 This study reports the first experimental evidence of using DNA as a polymeric additive to enhance protein crystallization. Using three kinds of DNA with different molecular weights—calf DNA, salmon DNA, and herring DNA—this study showed an ...
Bo Zhang +8 more
doaj +1 more source
Hematopoietic (stem) cells—The elixir of life?
FEBS Letters, EarlyView.The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo +4 more
wiley +1 more source
IUCrJ, 2017 The limiting factor in protein crystallography is still the production of high-quality crystals. In this regard, the authors have recently introduced hexatungstotellurate(VI) (TEW) as a new crystallization additive, which proved to be successful within ...
Christian Molitor +2 more
doaj +1 more source
Selecting temperature for protein crystallization screens using the temperature dependence of the second virial coefficient. [PDF]
PLoS ONE, 2011 Protein crystals usually grow at a preferable temperature which is however not known for a new protein. This paper reports a new approach for determination of favorable crystallization temperature, which can be adopted to facilitate the crystallization ...
Jun Liu +6 more
doaj +1 more source
Crystallization and preliminary crystallographic analysis of dUTPase from the helper phage Φ11 of Staphylococcus aureus [PDF]
, 2011 Staphylococcus aureus superantigen-carrying pathogenicity islands (SaPIs) have a determinant role in spreading virulence genes among bacterial populations that constitute a major health hazard.
Bendes, Ábris +5 more
core +1 more source
Biomolecular membrane protein crystallization [PDF]
Philosophical Magazine, 2012 Integral membrane proteins comprise approximately 30% of the sequenced genomes, and there is an immediate need for their high-resolution structural information. Currently, the most reliable approach to obtain these structures is x-ray crystallography. However, obtaining crystals of membrane proteins that diffract to high resolution appears to be quite ...
Jani Reddy, Bolla +2 more
openaire +2 more sources