Results 81 to 90 of about 178,624 (341)

Introduction to protein crystallization [PDF]

Methods, 2004
Biological macromolecules can be crystallized by a variety of techniques, and using a wide range of reagents which produce supersaturated mother liquors. These may, in turn, be applied under different physical conditions such as temperature. The fundamental approaches to devising successful crystallization conditions and the factors that influence them
openaire   +4 more sources

Functional Materials for Environmental Energy Harvesting in Smart Agriculture via Triboelectric Nanogenerators

Advanced Functional Materials, EarlyView.
This review explores functional and responsive materials for triboelectric nanogenerators (TENGs) in sustainable smart agriculture. It examines how particulate contamination and dirt affect charge transfer and efficiency. Environmental challenges and strategies to enhance durability and responsiveness are outlined, including active functional layers ...
Rafael R. A. Silva, Giulio Fatti, Emanuel Carlos, Guilherme Ferreira, Sumita Goswami, Suman Nandy, Luiz H. C. Mattoso, Elvira Fortunato, Caio G. Otoni, Rodrigo Martins   +9 more
wiley   +1 more source

The Solubility of Hen Lysozyme in Ethylammonium Nitrate/H2O Mixtures and a Novel Approach to Protein Crystallization

Molecules, 2010
We report on the solubility of hen lysozyme (HEWL) in aqueous ethylammonium nitrate (EAN) as a function of water content. We find the solubility behavior to be complex, exhibiting both a maximum (400 mg/mL) at very high EAN content) and a minimum at ...
C. Austen Angell, Nolene Byrne
doaj   +1 more source

The Role of Cations and Anions in the Formation of Crystallization Oligomers in Protein Solutions as Revealed by Combination of Small-Angle X-ray Scattering and Molecular Dynamics

Crystals, 2022
As is known from molecular dynamics simulation, lysozyme oligomers in crystallization solutions are most stable when taking into account as many precipitant ions as possible embedded in the corresponding crystal structure.
Margarita A. Marchenkova, Petr V. Konarev, Yuliya V. Kordonskaya, Kseniia B. Ilina, Yury V. Pisarevsky, Alexander V. Soldatov, Vladimir I. Timofeev, Mikhail V. Kovalchuk   +7 more
doaj   +1 more source

Crystallization of Adenylylsulfate Reductase from Desulfovibrio gigas: A Strategy Based on Controlled Protein Oligomerization [PDF]

, 2011
Adenylylsulfate reductase (adenosine 5′-phosphosulfate reductase, APS reductase or APSR, E.C.1.8.99.2) catalyzes the conversion of APS to sulfite in dissimilatory sulfate reduction. APSR was isolated and purified directly from massive anaerobically grown
Chan, Sunney I., Chen, Chun-Jung, Chiang, Yuan-Lan, Chuankhayan, Phimonphan, Fang, Jou-Yin, Hsieh, Yin-Cheng, Huang, Yen-Chieh, Liu, Ming-Yih, Wang, Vincent C.-C., Yang, Ming-Chi   +9 more
core   +1 more source

Introduction to protein crystallization [PDF]

Acta Crystallographica Section F Structural Biology Communications, 2013
Protein crystallization was discovered by chance about 150 years ago and was developed in the late 19th century as a powerful purification tool and as a demonstration of chemical purity. The crystallization of proteins, nucleic acids and large biological complexes, such as viruses, depends on the creation of a solution that is supersaturated in the ...
McPherson, Alexander, Gavira, Jose A
openaire   +4 more sources

Multifunctional Protein Eutectogels Based on Polyphenolic: Zwitterionic Low‐Transition‐Temperature Mixtures

Advanced Functional Materials, EarlyView.
This work presents the first example of multifunctional protein eutectogels based on a tannic acid: betaine low‐transition‐temperature mixture, exhibiting elastomeric behavior, strong adhesion to various substrates, including biological tissues, and notable anti‐inflammatory properties derived from the natural polyphenol and zwitterion.
Sergio Martin‐Saldaña, Alberto de León, María de la Mata, Nikolaos Politakos, Jorge L. Olmedo‐Martínez, Alejandro J. Müller, Ana Beloqui, Marcelo Calderón, Matias L. Picchio   +8 more
wiley   +1 more source

JINXED: just in time crystallization for easy structure determination of biological macromolecules

IUCrJ, 2023
Macromolecular crystallography is a well established method in the field of structural biology and has led to the majority of known protein structures to date.
Alessandra Henkel, Marina Galchenkova, Julia Maracke, Oleksandr Yefanov, Bjarne Klopprogge, Johanna Hakanpää, Jeroen R. Mesters, Henry N. Chapman, Dominik Oberthuer   +8 more
doaj   +1 more source

Insertion of T4-lysozyme (T4L) can be a useful tool for studying olfactory-related GPCRs. [PDF]

, 2012
The detergents used to solubilize GPCRs can make crystal growth the rate-limiting step in determining their structure. The Kobilka laboratory showed that insertion of T4-lysozyme (T4L) in the 3rd intracellular loop is a promising strategy towards ...
Baaske, Baud, Brian L. Cook, Cherezov, Chien, Christoph J. Wienken, Cook, Cook, Corin, Corin, Dahoun, Dieter Braun, Duhr, Garavito, Gimelbrant, Gimelbrant, Greenfield, Hanson, Horst Pick, Horst Vogel, Jaakola, Jacquier, Jayadev, Karolina Corin, Katada, Loll, Lu, Lu, McClintock, Philipp Baaske, Pick, Rasmussen, Ren, Rodriguez, Shimamura, Shirokova, Shuguang Zhang, Spehr, Spehr, Stefan Duhr, Wallin, Warne, Wienken, Wu   +43 more
core   +1 more source

pH‐Tunable Material Properties of Glycine‐Rich Condensates from Tick Bioadhesive

Advanced Functional Materials, EarlyView.
This work studies the influence of pH on the phase separation behavior of a disordered glycine‐rich protein found in tick bioadhesive. The results show profound impact on the propensity of coacervation, condensate microstructure and viscosity, amphiphilicity of the peptides, and effective encapsulation of therapeutic molecules.
Manali Nandy, Udit Ghosh, Ketan A. Ganar, Hans Ippel, Ingrid Dijkgraaf, Siddharth Deshpande   +5 more
wiley   +1 more source