Results 91 to 100 of about 441,330 (301)

Evaluation of anti-inflammatory effects of green tea and black tea: A comparative in vitro study

Journal of Advanced Pharmaceutical Technology & Research, 2012
The present study was conducted to evaluate and compare the anti-inflammatory effects of aqueous extracts of green tea and black tea leaves (Camellia sinensis) against the denaturation of protein in vitro.
Priyanka Chatterjee, Sangita Chandra, Protapaditya Dey, Sanjib Bhattacharya   +3 more
doaj   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

FEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava, Anoop Saraya, Deepak Gunjan, Rinu Sharma   +3 more
wiley   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Real-time protein unfolding: a method for determining the kinetics of native protein denaturation using a quantitative real-time thermocycler

BioTechniques, 2012
Protein stability can be monitored by many different techniques. However, these protocols are often lengthy, consume large amounts of protein, and require expensive and specialized instruments.
Kyle K. Biggar, Neal J. Dawson, Kenneth B. Storey   +2 more
doaj   +1 more source

Polar residues in the protein core of Escherichia coli thioredoxin are important for fold specificity [PDF]

, 2001
Most globular proteins contain a core of hydrophobic residues that are inaccessible to solvent in the folded state. In general, polar residues in the core are thermodynamically unfavorable except when they are able to form intramolecular hydrogen bonds ...
Bolon, Daniel N., Mayo, Stephen L.
core   +1 more source

New Powerful Protein Denaturant [PDF]

Biophysical Journal, 2013
Traditional denaturants such as urea and guanidinium hydrochloride effectively unfold a variety of proteins in an “all-or-none” fashion. However their high working concentration in combination with the strong absorption below 210 nm make it impossible to measure high quality circular dichroism spectra, which are commonly used to detect changes in ...
Perlova, Tatyana, Gelman, Hannah, Gruebele, Martin   +2 more
openaire   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Zein‐Based Adhesives: Sustainable Extraction and Application in Bioadhesive Technologies

Advanced Engineering Materials, EarlyView.
Zein is extracted from corn gluten meal using a simple and scalable process with high yield (~90%). The resulting protein is applied in bioadhesives modified with Ca2+ and Fe3+ ions, exhibiting substrate‐dependent adhesion. The findings demonstrate competitive bonding performance and highlight the role of ionic interactions in tuning adhesion ...
Paula Bertolino Sanvezzo, Daniela Winsley Valereto Friozi, Vinícius de Oliveira, Marcia Cristina Branciforti   +3 more
wiley   +1 more source

ORIGINAL ARTICLE: Membrane Stabilizing Activity and Protein Denaturation: A Possible Mechanism of Action for the Anti-Inflammatory Activity of Phyllanthus amarus [PDF]

Journal of Krishna Institute of Medical Sciences University, 2012
Background: Previous studies confirm the anti-inflammatory potential of Phyllanthus amarus herb. Aim and objective: Primary aim of the present study was to investigate the possible anti-inflammatory mechanism of Phyllanthus amarus extract using in-vitro ...
Atul R. Chopade, Prakash M. Somade, Fahim J. Sayyad   +2 more
doaj  

Hyperthermophile protein behavior: partially-structured conformations of Pyrococcus furiosus rubredoxin monomers generated through forced cold-denaturation and refolding. [PDF]

PLoS ONE, 2014
Some years ago, we showed that thermo-chemically denatured, partially-unfolded forms of Pyrococcus furiosus triosephosphateisomerase (PfuTIM) display cold-denaturation upon cooling, and heat-renaturation upon reheating, in proportion with the extent of ...
Sanjeev Kumar Chandrayan, Satya Prakash, Shubbir Ahmed, Purnananda Guptasarma   +3 more
doaj   +1 more source