Results 71 to 80 of about 4,548,844 (251)

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

FEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li, Miao Xin, Xiao‐wen Cui, Shi‐yin Yu, Fei Meng, Min Cao, Xiao‐hui Xiong, Yi‐chen Lu   +7 more
wiley   +1 more source

ORIGINAL ARTICLE: Membrane Stabilizing Activity and Protein Denaturation: A Possible Mechanism of Action for the Anti-Inflammatory Activity of Phyllanthus amarus [PDF]

Journal of Krishna Institute of Medical Sciences University, 2012
Background: Previous studies confirm the anti-inflammatory potential of Phyllanthus amarus herb. Aim and objective: Primary aim of the present study was to investigate the possible anti-inflammatory mechanism of Phyllanthus amarus extract using in-vitro ...
Atul R. Chopade, Prakash M. Somade, Fahim J. Sayyad   +2 more
doaj  

Tyrosine Flourescence to Monitor the Denaturation of a Bacterial Protein [PDF]

, 1992
Tyrosine fluorescence was used to monitor the denaturation of a protein. The protein used as our model system was the regulatory subunit (RSU) of aspartate transcarbamoylase from E. coli. RSU is a dimer of two identical chains with one zinc ion bound per
Trudeau, Kristin L.
core  

Analyzing Protein Denaturation using Fast Differential Scanning Calorimetry

, 2012
This paper investigates the possibility to measure protein denaturation with Fast Differential Scanning Calorimetry (FDSC). Cancer can be diagnosed by measuring protein denaturation in blood plasma using Differential Scanning Calorimetry (DSC).
Iervolino, E. (author), Vanden Poel, G. (author), Istrate, D. (author), Splinter, R. (author), Van Herwaarden, A.W. (author), Sarro, P.M. (author)   +5 more
core   +1 more source

GBS‐MeDIP: A method for the simultaneous identification of genetic and epigenetic variants in tumors

FEBS Open Bio, EarlyView.
GBS‐MeDIP combines DNA sequencing with enrichment of methylated DNA to profile genetic and epigenetic variation in the same reduced genomic fraction. Applied to gastric cancer and parathyroid tumors, it identifies sequence variants, copy number changes, and differentially methylated regions together, helping distinguish true epigenetic changes from ...
Fábio Pértille, Shiva Rezaei, Julia Uffenorde, Oliver Gimm, Mohammad Ali Hosseinpour Feizi, Luiz Lehmann Coutinho, Carlos Guerrero‐Bosagna   +6 more
wiley   +1 more source

Influence of Boiling, Steaming and Frying of Selected Leafy Vegetables on the In Vitro Anti-inflammation Associated Biological Activities

Plants, 2018
The aim of the present study was to evaluate the effect of cooking (boiling, steaming, and frying) on anti-inflammation associated properties in vitro of six popularly consumed green leafy vegetables in Sri Lanka, namely: Centella asiatica, Cassia ...
K. D. P. P. Gunathilake, K. K. D. S. Ranaweera, H. P. V. Rupasinghe   +2 more
doaj   +1 more source

Influence of pH and ionic strength on heat-induced formation and rheological properties of soy protein gels in relation to denaturation and their protein compositions

, 2002
The influence of pH and ionic strength on gel formation and gel properties of soy protein isolate (SPI) in relation to denaturation and protein aggregation/precipitation was studied.
Vliet, T. van, Renkema, J.M.S., Gruppen, H.   +2 more
core  

Quantitative Fluorescence Spectral Analysis of Protein Denaturation [PDF]

, 2013
This chapter describes a procedure of global analysis of the steady-state spectra measured with different concentrations of the denaturant to quantitatively study protein denaturation. With the help of physicochemical models, relevant spectral parameters
Laptenok, S., van Stokkum, I.H.M.; id_orcid, Sergey P. Laptenok, Ivo H. M. van Stokkum   +3 more
core   +1 more source

Blood RNA Biomarker Signatures for Early Diagnosis and Prognosis in Ischemic and Hemorrhagic Stroke: The IBIS‐CT1 Study

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the expression of nine blood RNA biomarkers in a clinical trial based on genes previously identified in an experimental monkey model of stroke for diagnosis feasibility and prognostication. Methods IBIS‐CT1 was a prospective longitudinal study enrolling patients with ischemic stroke (IS) or intracerebral hemorrhage (ICH ...
Salomé Retailleau, Marie Bruguet, Irina Viakhireva‐Dovganyuk, François‐Mathias Merrien, Denis Marechal, Aurore Jourdain, Philippe Goas, Jordan Coris, Julien Asselineau, Maëlys Consigny, Gérald Le Gac, Serge Timsit   +11 more
wiley   +1 more source

Systematic FTIR Spectroscopy Study of the Secondary Structure Changes in Human Serum Albumin under Various Denaturation Conditions

Biomolecules, 2019
Human serum albumin (HSA) is the most abundant protein in blood plasma. HSA is involved in the transport of hormones, fatty acids, and some other compounds, maintenance of blood pH, osmotic pressure, and many other functions.
Dmitrii Usoltsev, Vera Sitnikova, Andrey Kajava, Mayya Uspenskaya   +3 more
doaj   +1 more source