Results 71 to 80 of about 295,099 (303)

Denaturation of the SARS-CoV-2 spike protein under non-thermal microwave radiation

Scientific Reports, 2021
SARS-CoV-2, the virus that causes COVID-19, is still a widespread threat to society. The spike protein of this virus facilitates viral entry into the host cell.
Pooya Afaghi, Michael Anthony Lapolla, Khashayar Ghandi   +2 more
doaj   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Differences in the pathways of proteins unfolding induced by urea and guanidine hydrochloride: molten globule state and aggregates. [PDF]

PLoS ONE, 2010
It was shown that at low concentrations guanidine hydrochloride (GdnHCl) can cause aggregation of proteins in partially folded state and that fluorescent dye 1-anilinonaphthalene-8-sulfonic acid (ANS) binds with these aggregates rather than with ...
Olga I Povarova, Irina M Kuznetsova, Konstantin K Turoverov   +2 more
doaj   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Effect of Lactobacillus pentosus fermentation on molecular structure and gel quality of peanut protein

Journal of Future Foods
Peanut protein was fermented by Lactobacillus pentosus, to study the effects of different protein composition of peanut protein isolate, arachin and concomitant arachin on their molecular structure and thermal properties during fermentation, the ...
Wenjun Li, Yexia Guan, Shumin Chen, Xiaoxuan Du, Beibei Yan, Zixu Wang, Runze Ma, Yunxin Zhang, Huang Huang, Deyuan Li, Wei Li   +10 more
doaj   +1 more source

Evaluation of anti-inflammatory effects of green tea and black tea: A comparative in vitro study

Journal of Advanced Pharmaceutical Technology & Research, 2012
The present study was conducted to evaluate and compare the anti-inflammatory effects of aqueous extracts of green tea and black tea leaves (Camellia sinensis) against the denaturation of protein in vitro.
Priyanka Chatterjee, Sangita Chandra, Protapaditya Dey, Sanjib Bhattacharya   +3 more
doaj   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Monitoring protein denaturation using thermal conductivity probe

, 2018
We propose a method for probing denaturation of proteins by measuring the thermal conductivity of the solution. We use the three-omega method with a microfabricated ac thermal sensor to measure the thermal conductivity of lysozyme, beta-lactoglobulin ...
Byoung kyoo Park, Namwoo Yi, Kim, D, Park, J   +3 more
core   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Analyzing Protein Denaturation using Fast Differential Scanning Calorimetry

, 2012
This paper investigates the possibility to measure protein denaturation with Fast Differential Scanning Calorimetry (FDSC). Cancer can be diagnosed by measuring protein denaturation in blood plasma using Differential Scanning Calorimetry (DSC).
Splinter, R., Iervolino, E. (author), Istrate, D., Vanden Poel, G. (author), Iervolino, E., Istrate, D. (author), Van Herwaarden, A.W., Vanden Poel, G., Splinter, R. (author), Van Herwaarden, A.W. (author), Poel, G. Vanden, Sarro, P.M. (author), Sarro, P.M.   +12 more
core   +1 more source