Results 11 to 20 of about 2,066,004 (263)

Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation

Nature Communications, 2023
Biomolecular condensates formed via phase separation of proteins and nucleic acids are thought to be associated with a wide range of cellular functions and dysfunctions.
Ashish Joshi, Anuja Walimbe, Anamika Avni, Sandeep K. Rai, Lisha Arora, Snehasis Sarkar, Samrat Mukhopadhyay   +6 more
doaj   +1 more source

Spatiotemporal modulations in heterotypic condensates of prion and α-synuclein control phase transitions and amyloid conversion

Nature Communications, 2022
The authors show that prion protein and α-synuclein undergo phase separation through domain-specific electrostatic interactions. These complex coacervates possess electrostatic nanoclusters and can convert into multiphasic condensates and amyloids.
Aishwarya Agarwal, Lisha Arora, Sandeep K. Rai, Anamika Avni, Samrat Mukhopadhyay   +4 more
doaj   +1 more source

Single-droplet surface-enhanced Raman scattering decodes the molecular determinants of liquid-liquid phase separation

Nature Communications, 2022
The authors introduce a unique single-droplet surface-enhanced Raman scattering (SERS) methodology that illuminates a wealth of molecular information within the mesoscopic liquid condensed phase of Fused in Sarcoma in the absence and presence of RNA.
Anamika Avni, Ashish Joshi, Anuja Walimbe, Swastik G. Pattanashetty, Samrat Mukhopadhyay   +4 more
doaj   +1 more source

Consensus protein design [PDF]

Protein Engineering Design and Selection, 2016
A popular and successful strategy in semi-rational design of protein stability is the use of evolutionary information encapsulated in homologous protein sequences. Consensus design is based on the hypothesis that at a given position, the respective consensus amino acid contributes more than average to the stability of the protein than non-conserved ...
Porebski, Benjamin T., Buckle, Ashley M.
openaire   +2 more sources

Adjuvanting a subunit SARS-CoV-2 vaccine with clinically relevant adjuvants induces durable protection in mice

npj Vaccines, 2022
Adjuvants enhance the magnitude and the durability of the immune response to vaccines. However, there is a paucity of comparative studies on the nature of the immune responses stimulated by leading adjuvant candidates.
Lilit Grigoryan, Audrey Lee, Alexandra C. Walls, Lilin Lai, Benjamin Franco, Prabhu S. Arunachalam, Yupeng Feng, Wei Luo, Abigail Vanderheiden, Katharine Floyd, Samuel Wrenn, Deleah Pettie, Marcos C. Miranda, Elizabeth Kepl, Rashmi Ravichandran, Claire Sydeman, Natalie Brunette, Michael Murphy, Brooke Fiala, Lauren Carter, Robert L. Coffman, David Novack, Harry Kleanthous, Derek T. O’Hagan, Robbert van der Most, Jason S. McLellan, Mehul Suthar, David Veesler, Neil P. King, Bali Pulendran   +29 more
doaj   +1 more source

Antibody Profiling: Kinetics with Native Biomarkers for Diagnostic Assay and Drug Developments

Biosensors, 2023
Despite remarkable progress in applied Surface Plasmon Resonance (SPR)-based methods, concise monitoring of kinetic properties for native biomarkers from patient samples is still lacking.
Ute Jucknischke, Sebastian Friebe, Markus Rehle, Laura Quast, Sven H. Schmidt   +4 more
doaj   +1 more source

Single-chain dimers from de novo immunoglobulins as robust scaffolds for multiple binding loops

Nature Communications, 2023
Antibody derivatives have sought to recapitulate the antigen binding properties of antibodies, but with improved biophysical attributes convenient for therapeutic, diagnostic and research applications.
Jorge Roel-Touris, Marta Nadal, Enrique Marcos   +2 more
doaj   +1 more source

Structure-based design of stabilized recombinant influenza neuraminidase tetramers

Nature Communications, 2022
Influenza virus neuraminidase (NA) is a drug target and a potential vaccine antigen. Here, the authors provide a detailed analysis of the conformational stability of NA, and show how expression and stability of recombinant NA antigens can be strengthened
Daniel Ellis, Julia Lederhofer, Oliver J. Acton, Yaroslav Tsybovsky, Sally Kephart, Christina Yap, Rebecca A. Gillespie, Adrian Creanga, Audrey Olshefsky, Tyler Stephens, Deleah Pettie, Michael Murphy, Claire Sydeman, Maggie Ahlrichs, Sidney Chan, Andrew J. Borst, Young-Jun Park, Kelly K. Lee, Barney S. Graham, David Veesler, Neil P. King, Masaru Kanekiyo   +21 more
doaj   +1 more source

Novel multivalent design of a monoclonal antibody improves binding strength to soluble aggregates of amyloid beta

Translational Neurodegeneration, 2021
Background Amyloid-β (Aβ) immunotherapy is a promising therapeutic strategy in the fight against Alzheimer’s disease (AD). A number of monoclonal antibodies have entered clinical trials for AD. Some of them have failed due to the lack of efficacy or side-
Fadi Rofo, Jos Buijs, Ronny Falk, Ken Honek, Lars Lannfelt, Anna M. Lilja, Nicole G. Metzendorf, Tobias Gustavsson, Dag Sehlin, Linda Söderberg, Greta Hultqvist   +10 more
doaj   +1 more source

De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8

Nature Communications, 2023
The RGD (Arg-Gly-Asp)-binding integrins αvβ6 and αvβ8 are clinically validated cancer and fibrosis targets of considerable therapeutic importance.
Anindya Roy, Lei Shi, Ashley Chang, Xianchi Dong, Andres Fernandez, John C. Kraft, Jing Li, Viet Q. Le, Rebecca Viazzo Winegar, Gerald Maxwell Cherf, Dean Slocum, P. Daniel Poulson, Garrett E. Casper, Mary L. Vallecillo-Zúniga, Jonard Corpuz Valdoz, Marcos C. Miranda, Hua Bai, Yakov Kipnis, Audrey Olshefsky, Tanu Priya, Lauren Carter, Rashmi Ravichandran, Cameron M. Chow, Max R. Johnson, Suna Cheng, McKaela Smith, Catherine Overed-Sayer, Donna K. Finch, David Lowe, Asim K. Bera, Gustavo Matute-Bello, Timothy P. Birkland, Frank DiMaio, Ganesh Raghu, Jennifer R. Cochran, Lance J. Stewart, Melody G. Campbell, Pam M. Van Ry, Timothy Springer, David Baker   +39 more
doaj   +1 more source