Results 91 to 100 of about 765,059 (313)

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Colorful protein-based fluorescent probes for collagen imaging [PDF]

, 2014
Real-time visualization of collagen is important in studies on tissue formation and remodeling in the research fields of developmental biology and tissue engineering.
Aper, SJA Stijn, Turnhout, MC Mark van, Spreeuwel, ACC Ariane van, Merkx, M., Zon, van der, N.L.L., Rambelje, de la, S.L., Bouten, C.V.C., Bouten, CVC Carlijn, Pieters, PA Pascal, Merkx, M Maarten, Turnhout, van, M.C.; id_orcid, Linden, AJ Ardjan van der, Rambelje, SL de la, Turnhout, van, M.C., Spreeuwel, van, A.C.C., Bouten, C.V.C.; id_orcid, Aper, S.J.A., Zon, NLL van der, van der Linden, A.J., Pieters, P.A.   +19 more
core   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Insights from engineering the Affibody-Fc interaction with a computational-experimental method

, 2017
The interaction between the Staphylococcal Protein A (SpA) domain B (the basis of the Affibody) molecule and the Fc of IgG is key to the use of Affibodies in affinity chromatography and in potential therapies against certain inflammatory diseases ...
Danielson, U. Helena,, Dourado, Daniel F A R, Sara Solbak, Flores, Samuel Coulbourn, Housaindokht, Mohammad Reza,, Masoumeh Nosrati, Anders Virtanen, U Helena Danielson, Solbak, Sara,, Nosrati, Masoumeh,, Solbak, Sara, Ken G Andersson, Virtanen, Anders, Dourado, Daniel F. A. R.,, Flores, Samuel Coulbourn,, Nissbeck, Mikael, Andersson, Ken G,, Löfblom, John,, Samuel Coulbourn Flores, Virtanen, Anders,, Danielson, U Helena, Andersson, Ken G.,, Nissbeck, Mikael,, Nosrati, Masoumeh, Daniel F A R Dourado, Nordesjö, Olle, Mikael Nissbeck, Olle Nordesjö, Mohammad Reza Housaindokht, John Löfblom, Housaindokht, Mohammad Reza, Nordesjö, Olle,   +31 more
core   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Protein engineering for therapeutics.

, 2012
"The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and ...
Wittrup, K. Dane., Verdine, Gregory L.(Gregory Lawrence)   +1 more
core  

Protein engineering of cytochrome c by semisynthesis: substitutions at glutamic acid 66

, 1986
We have used protein semisynthesis to prepare four analogues of horse cytochrome c, in which the glutamic acid residue at position 66 has been removed and replaced by norvaline, glutamine, lysine and, as a methodological control, glutamic acid.
Wallace, C. J., Corthesy, B. E.
core   +1 more source

Auxin-mediated protein depletion for metabolic engineering in terpene-producing yeast

, 2021
In metabolic engineering, loss-of-function experiments are used to understand and optimise metabolism. A conditional gene inactivation tool is required when gene deletion is lethal or detrimental to growth.
Lu, Zeyu, Peng, Bingyin, Dumsday, Geoff, Ebert, Birgitta E, Vickers, Claudia E   +4 more
core   +1 more source

Correction: Galectins use N-glycans of FGFs to capture growth factors at the cell surface and fine-tune their signaling

Cell Communication and Signaling, 2023
Aleksandra Gedaj, Dominika Zukowska, Natalia Porebska, Marta Pozniak, Mateusz Krzyscik, Aleksandra Czyrek, Daniel Krowarsch, Malgorzata Zakrzewska, Jacek Otlewski, Lukasz Opalinski   +9 more
doaj   +1 more source

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source