Results 171 to 180 of about 1,281,585 (240)

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

FEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

Critical Assessment of Protein Engineering (CAPE): A Student Challenge on the Cloud. [PDF]

ACS Synth Biol
Fu L, Gao Y, Chen Y, Wang Y, Fang X, Tian S, Dong H, Zhang Y, Chen Z, Wang Z, Hu S, Yi X, Si T.   +12 more
europepmc   +1 more source

The role of lipid metabolism in neuronal senescence

FEBS Open Bio, EarlyView.
Disrupted lipid metabolism, through alterations in lipid species or lipid droplet accumulation, can drive neuronal senescence. However, lipid dyshomeostasis can also occur alongside neuronal senescence, further amplifying tissue damage. Delineating how lipid‐induced senescence emerges in neurons and glial cells, and how it contributes to ageing and ...
Dikaia Tsagkari, Eleftheria Panagiotidou, Nektarios Tavernarakis   +2 more
wiley   +1 more source

Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O. [PDF]

Chembiochem
Möller C, Terholsen H, Schmöker O, Lê TLA, Wesche J, Schmiade P, Eppendorfer E, Rimkus N, Girbardt B, Böttcher D, Palm GJ, Hoppen J, Lammers M, Greinacher A, Aurich K, Bornscheuer UT.   +15 more
europepmc   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Semirational Protein Engineering Yields Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators with Enhanced Brightness and Red Shifted Absorption Bands. [PDF]

Chem Bio Eng
Nikolaev DM, Metelkina EM, Domskii NA, Osadchy IR, Mereshchenko AS, Bondarev SA, Zhouravleva GA, Shtyrov AA, Panov MS, Ryazantsev MN.   +9 more
europepmc   +1 more source

Understanding bio‐based polymers: A study of origins, properties, biodegradation and their impact on health and the environment

FEBS Open Bio, EarlyView.
This review provides an overview of bio‐based polymer sources, their unique functional properties and their environmental impact, and addresses their role as sustainable alternatives. It discusses end‐of‐life options, including composting and anaerobic digestion for renewable energy.
Sabina Kolbl Repinc, Blaž Stres, Mirica Karlovits, Igor Karlovits, Petra Jerič, Ondrej Panák, Anja Verbič, Blaž Likozar, Uroš Novak   +8 more
wiley   +1 more source

Structural studies of the IFNλ4 receptor complex using cryoEM enabled by protein engineering. [PDF]

Nat Commun
Grubbe WS, Zhang B, Kauffman A, Byléhn F, Padoł K, Jung HG, Park SB, Priest JM, Özkan E, de Pablo JJ, Liang TJ, Zhao M, Mendoza JL.   +12 more
europepmc   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Chemical Protein Engineering: Backbone Cyclization Rescues Folding of a 183-Residue Truncated Domain of Malaria Parasite Protein PfAMA1. [PDF]

Chemistry
Mannuthodikayil J, Malik V, Kar A, Singh S, Mandal K.   +4 more
europepmc   +1 more source