Results 191 to 200 of about 1,300,384 (288)

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

FEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamoto   +6 more
wiley   +1 more source

Functional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering. [PDF]

Appl Microbiol Biotechnol
Patti S, De Rose SA, Isupov MN, Magrini Alunno I, Riva S, Ferrandi EE, Littlechild JA, Monti D.   +7 more
europepmc   +2 more sources

Enhanced rational protein engineering to reduce viscosity in high-concentration IgG1 antibody solutions. [PDF]

MAbs
Lefevre TJ, Caldwell JG, Gallegos A, Du Q, Houston E, Kaplan G, Esfandiary R.   +6 more
europepmc   +1 more source

Protein engineering by chemical means? [PDF]

, 2017
Offord, R.E.
core  

Pioglitazone plus (−)‐epigallocatechin gallate: a novel approach to enhance osteogenic performance in aged bone marrow mesenchymal stem cells

FEBS Open Bio, EarlyView.
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen, Jia‐Ci Jhang, Chun‐Yi Peng, Quốc Cường Nguyễn, Feng‐Huei Lin, Hung‐Ming Wu, Shankung Lin   +6 more
wiley   +1 more source

Results of the Protein Engineering Tournament: An Open Science Benchmark for Protein Modeling and Design. [PDF]

Proteins
Armer C, Kane H, Cortade DL, Redestig H, Estell DA, Yusuf A, Rollins N, Spinner A, Marks D, Brunette TJ, Kelly PJ, DeBenedictis E.   +11 more
europepmc   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

PRIME, a temperature-guided language model revolutionizes protein engineering. [PDF]

Acta Pharm Sin B
Yu Y, Wang Q, Zou Y.
europepmc   +1 more source

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

FEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

An update on thermostable keratinases for protein engineering against feather pollutants. [PDF]

Appl Microbiol Biotechnol
J L BJ, Dhanasingh I.
europepmc   +1 more source