Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Investigation of Few-Layer Graphene–Ubiquitin Interactions with Optical Spectroscopy Techniques
Understanding the molecular mechanisms of protein–nanoparticle interactions is crucial for enabling the development of new applications in biomedicine and nanotechnology.
Burcu Gencay, Günnur Güler
doaj +1 more source
Molecular determinants of signal transduction in tropomyosin receptor kinases
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley +1 more source
Novel antioxidant peptide from tilapia skin: identification and structure elucidation
In recent years, a variety of antioxidant peptides have been isolated and purified from diverse food sources. These peptides have attracted significant interest due to their potential therapeutic benefits in managing cardiovascular diseases and their ...
Ye-Liu Fu, Cheng-Ri Yin, Mei Shi, Na Li
doaj +1 more source
Les mécanismes de repliement des protéines solubles [PDF]
Mechanisms of folding of soluble proteins. The function of a protein is carried out by its three-dimensional structure. The rules for translation of the information encoded by DNAinto an amino acid sequence are well known.
Brasseur B., Benhabilès N., Thomas A.
doaj
Comparative analysis of chloroplast genomes from 14 genera of Thymelaeaceae revealed variation in gene content, ranging from 128 to 142 genes, primarily influenced by IR expansion/contraction events and pseudogenization of ndhF, ndhI, and ndhG. Two large inversions were detected within the large single‐copy region, including a synapomorphic inversion ...
Abdullah +8 more
wiley +1 more source
Engineered, highly reactive substrates of microbial transglutaminase enable protein labeling within various secondary structure elements [PDF]
Natalie M. Rachel +4 more
openalex +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
SSDraw: Software for generating comparative protein secondary structure diagrams. [PDF]
Chen EA, Porter LL.
europepmc +1 more source
Using circular dichroism spectra to estimate protein secondary structure
N. Greenfield
semanticscholar +1 more source

