Results 81 to 90 of about 220,067 (314)
Bacterial Microcolonies in Gel Beads for High-throughput Screening
Bio-Protocol, 2018 High-throughput screening of a DNA library expressed in a bacterial population for identifying potentially rare members displaying a property of interest is a crucial step for success in many experiments such as directed evolution of proteins and ...
Yolanda Schaerli
doaj +1 more source
Proceedings of the National Academy of Sciences, 1998 In epithelial cells, sorting of membrane proteins to the basolateral surface depends on the presence of a ba solateral s orting s ignal (BaSS) in their cytoplasmic domain.
P, Zheng +3 more
openaire +3 more sources
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Myelination: all about Rac n roll. [PDF]
, 2007 During the development of the peripheral nervous system, Schwann cells select individual axons from a nerve bundle and establish a one-to-one relationship through a process termed radial sorting. Recent findings identify the Rho family GTPase Rac1 as the
Chan, Jonah
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Aggressive prostate cancer is associated with pericyte dysfunction
Molecular Oncology, EarlyView.Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero +11 more
wiley +1 more source
Phosphoglycerate kinase 1 acts as a cargo adaptor to promote EGFR transport to the lysosome
Nature CommunicationsThe epidermal growth factor receptor (EGFR) plays important roles in multiple cellular events, including growth, differentiation, and motility. A major mechanism of downregulating EGFR function involves its endocytic transport to the lysosome. Sorting of
Shao-Ling Chu +6 more
doaj +1 more source
Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line
Skeletal Muscle, 2018 Background Satellite cells are residential muscle stem cells that express a paired box protein, PAX7. Results Here, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of ...
Yasuo Kitajima, Yusuke Ono
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Adaptor protein-3: A key player in RBL-2H3 mast cell mediator release. [PDF]
PLoS ONE, 2017 Mast cell (MC) secretory granules are Lysosome-Related Organelles (LROs) whose biogenesis is associated with the post-Golgi secretory and endocytic pathways in which the sorting of proteins destined for a specific organelle relies on the recognition of ...
Elaine Zayas Marcelino da Silva +5 more
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Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene [PDF]
, 2007 We aim to introduce the penicillin biosynthetic pathway into the methylotrophic yeast Hansenula polymorpha. To allow simultaneous expression of the multiple genes of the penicillin biosynthetic pathway, additional markers were required.
Gidijala, Loknath, +3 more
core +2 more sources
N‐glycans are not a universal signal for apical sorting of secretory proteins
FEBS Letters, 1999 In MDCK cells, N‐glycans have been shown to determine the sorting of secretory proteins and membrane proteins to the apical domain in the absence of a dominant basolateral targeting signal. We have examined the sorting of endogenous proteins in ECV304 cells in the presence and absence of tunicamycin, an inhibitor of N‐linked glycosylation.
Su, Tao, Cariappa, Rohit, Stanley, Keith
openaire +2 more sources