Results 71 to 80 of about 6,092,811 (364)

A histidine‐rich extension of the mitochondrial F0 subunit ATP6 from the ice worm Mesenchytraeus solifugus increases ATP synthase activity in bacteria

FEBS Letters, EarlyView.
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley, Daniel H. Shain, Eric A. Klein   +2 more
wiley   +1 more source

Modular decomposition of protein structure using community detection [PDF]

Journal of Complex Networks (2018), cny014, 2018
As the number of solved protein structures increases, the opportunities for meta-analysis of this dataset increase too. Protein structures are known to be formed of domains; structural and functional subunits that are often repeated across sets of proteins.
arxiv   +1 more source

Regulation of NHE3 activity by G protein subunits in renal brush-border membranes.

American Journal of Physiology. Regulatory Integrative and Comparative Physiology, 2000
NHE3 activity is regulated by phosphorylation/dephosphorylation processes and membrane recycling in intact cells. However, the Na(+)/H(+) exchanger (NHE) can also be regulated by G proteins independent of cytoplasmic second messengers, but the G protein ...
F. Albrecht, J. Xu, O. Moe, U. Hopfer, W. Simonds, J. Orlowski, P. Jose   +6 more
semanticscholar   +1 more source

Subunit counting in membrane-bound proteins [PDF]

Nature Methods, 2007
The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of interest.
Maximilian H. Ulbrich, Ehud Y. Isacoff
openaire   +2 more sources

Characteristics of the Kelch domain containing (KLHDC) subfamily and relationships with diseases

FEBS Letters, EarlyView.
The Kelch protein superfamily includes 63 members, with the KLHDC subfamily having 10 proteins. While their functions are not fully understood, recent advances in KLHDC2's structure and role in protein degradation have highlighted its potential for drug development, especially in PROTAC therapies.
Courtney Pilcher, Paula Armina V. Buco, Jia Q. Truong, Paul A. Ramsland, Monique F. Smeets, Carl R. Walkley, Jessica K. Holien   +6 more
wiley   +1 more source

Insertion of the FeB cofactor in cNORs lacking metal inserting chaperones

FEBS Letters, EarlyView.
Nitric oxide reductase is an enzyme found in the bacterial denitrification pathway. The NOR active site contains a non‐heme iron, often, but not always inserted with the assistance of chaperones. Here, we study the insertion of FeB in the subfamily of cNORs lacking chaperones and found a putative channel, conserved in the family, perhaps enabling the ...
Sofia Appelgren, Pia Ädelroth
wiley   +1 more source

CRISPR-Cas9 Arabidopsis mutants of genes for ARPC1 and ARPC3 subunits of ARP2/3 complex reveal differential roles of complex subunits

Scientific Reports, 2022
Protein complex Arp2/3 has a conserved role in the nucleation of branched actin filaments. It is constituted of seven subunits, including actin-like subunits ARP2 and ARP3 plus five other subunits called Arp2/3 Complex Component 1 to 5, which are not ...
Erica Bellinvia, Judith García-González, Petra Cifrová, Jan Martinek, Lenka Sikorová, Lenka Havelková, Kateřina Schwarzerová   +6 more
doaj   +1 more source

Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double‐stranded DNA

EMBO Journal, 1997
Group II introns use intron‐encoded reverse transcriptase, maturase and DNA endonuclease activities for site‐specific insertion into DNA. Remarkably, the endonucleases are ribonucleoprotein complexes in which the excised intron RNA cleaves the sense ...
Huatao Guo, S. Zimmerly, P. Perlman, A. Lambowitz   +3 more
semanticscholar   +1 more source

Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells

FEBS Letters, EarlyView.
Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi   +6 more
wiley   +1 more source

Interaction Between Motor Domains Can Explain the Complex Dynamics of Heterodimeric Kinesins [PDF]

, 2007
Motor proteins are active enzyme molecules that play a crucial role in many biological processes. They transform the chemical energy into the mechanical work and move unidirectionally along rigid cytoskeleton filaments. Single-molecule experiments suggest that motor proteins, consisting of two motor domains, move in a hand-over-hand mechanism when each
arxiv   +1 more source